LNK/SH2B3 Overexpression Lysate


Western Blot: LNK Overexpression Lysate (Adult Normal) [NBL1-15917] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LNK.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

LNK/SH2B3 Overexpression Lysate Summary

Transient overexpression lysate of SH2B adaptor protein 3 (SH2B3) The lysate was created in HEK293T cells, using Plasmid ID RC218359 and based on accession number NM_005475. The protein contains a C-MYC/DDK Tag.


Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LNK/SH2B3 Overexpression Lysate

  • IDDM20
  • LNK
  • LNKSH2B adapter protein 3
  • Lymphocyte adapter protein
  • Lymphocyte-specific adapter protein Lnk
  • SH2B adaptor protein 3
  • SH2B3
  • Signal transduction protein Lnk


T-cell activation requires stimulation of the T-cell receptor (TCR; see MIM 186880)-CD3 (see CD3Z; MIM 186780) complex, followed by recruitment of an array of intracellular signaling proteins (e.g., GRB2 (MIM 108355) and PLCG1 (MIM 172420)). Mediating the interaction between the extracellular receptors and intracellular signaling pathways are adaptor proteins such as LAT (MIM 602354), TRIM (MIM 604962), and LNK.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol SH2B3