LMW-PTP/ACP1 Antibody [HRP]

Images

 
Western Blot: LMW-PTP/ACP1 Antibody [HRP] [NB120-21017] - Lane 1: Acid Phosphatase (Potato). Load: 50 ng per lane. Primary antibody: Acid Phosphatase Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit ...read more

Product Details

Summary
Product Discontinued
View other related LMW-PTP/ACP1 Primary Antibodies

Order Details


    • Catalog Number
      NB120-21017
    • Availability
      Product Discontinued

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LMW-PTP/ACP1 Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Potato Acid Phosphatase
Localization
Cytoplasmic
Specificity
By immunoelectrophoresis, gives a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Potato Acid Phosphatase.
Clonality
Polyclonal
Host
Rabbit
Gene
ACP1
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
HRP
Format
HRP
Buffer
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
Preservative
0.01% Gentamicin Sulfate
Purity
Delipidation and Defibrination

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:20000
Readout System

Reactivity Notes

Cross-reacts with Potato. Not yet tested in other species.

Alternate Names for LMW-PTP/ACP1 Antibody [HRP]

  • acid phosphatase 1, soluble
  • acid phosphatase of erythrocyte
  • ACP1
  • Adipocyte acid phosphatase
  • cytoplasmic phosphotyrosyl protein phosphatase
  • EC 3.1.3.2
  • EC 3.1.3.48
  • HAAP
  • LMWPTP
  • LMW-PTP
  • LMW-PTP/ACP1
  • LMW-PTPase
  • Low molecular weight cytosolic acid phosphatase
  • low molecular weight phosphotyrosine protein phosphatase
  • MGC111030
  • MGC3499
  • protein tyrosine phosphatase
  • Red cell acid phosphatase 1

Background

Phosphatase enzymes catalyse hydrolysis of phosphoric acid esters of various alcohols, e.g. a hexose phosphate, to yield the alcohol and free inorganic phosphate. This may be a way of recycling phosphate in the cell, and the level of phosphate may be elevated under conditions of phosphate starvation (in algae for example). Acid phosphatases have pH optima below 7, whereas alkaline phosphatases are most active above pH 7. Commercial quantities of acid phosphatase are obtained from plant sources e.g. potato and wheat germ, whereas similar quantities of alkaline phosphatase are obtained from animal intestine and bacteria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol ACP1
Entrez

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