LMAN1 Antibody (1A8)

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Western Blot: LMAN1 Antibody (1A8) [NBP2-03381] Analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody (1A8) [NBP2-03381] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1.
Immunohistochemistry-Paraffin: LMAN1 Antibody (1A8) [NBP2-03381] - Staining of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (1A8) [NBP2-03381] - HEK293T cells transfected with either RC207088 overexpress plasmid(Red) or empty vector control plasmid(Blue) wereimmunostained by anti-LMAN1 antibodyand then analyzed ...read more
Western Blot: LMAN1 Antibody (1A8) [NBP2-03381] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: LMAN1 Antibody (1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (1A8) [NBP2-03381] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (1A8) [NBP2-03381] - Staining of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (1A8) [NBP2-03381] - Staining of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: LMAN1 Antibody (1A8) [NBP2-03381] - Staining of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody.
Flow Cytometry: LMAN1 Antibody (1A8) [NBP2-03381] - Analysis of Jurkat cells, using anti-LMAN1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

LMAN1 Antibody (1A8) Summary

Immunogen
Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LMAN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Publications
Read Publications using
NBP2-03381 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LMAN1 Antibody (1A8)

  • ERGIC-53
  • ERGIC53ER-Golgi intermediate compartment 53 kDa protein
  • F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53
  • FMFD1
  • Gp58
  • intracellular mannose specific lectin
  • Intracellular mannose-specific lectin MR60
  • Lectin mannose-binding 1
  • lectin, mannose-binding, 1
  • MCFD1coagulation factor V-factor VIII combined deficiency
  • MR60
  • protein ERGIC-53

Background

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LMAN1 Antibody (NBP2-03381)(2)

We have publications tested in 2 confirmed species: Human, Monkey.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
(1)
Monkey
(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LMAN1
Entrez