Western Blot: LIMPII/lpg85 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 ...read more
Immunocytochemistry/ Immunofluorescence: LIMPII/lpg85 Antibody [NB400-129] - Detection of LIMPII/lpg85 (Green) in Hela cells using NB400-129. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: LIMPII/lpg85 Antibody [NB400-129] - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.
This LIMPII/lpg85 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot, where a band is observed at ~85 kDa. Use in IHC-P was reported in scientific literature (PMID: 23863627). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
85 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Alternate Names for LIMPII/lpg85 Antibody
CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
LIMPIICD36 antigen-like 2
lysosome membrane protein 2
Lysosome membrane protein II
Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
scavenger receptor class B, member 2
LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for LIMPII/lpg85 Antibody (NB400-129). (Showing 1 - 1 of 1 FAQs).
Can you please inform me about the peptide used as immunogen? It is referred that is the a C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence, but the amino acid sequence used is not indicated.
NB400-129 was made to a sequence between amino acids 425-480.
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