LIMPII/lpg85 Antibody

Images

 
Western Blot: LIMPII/lpg85 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 ...read more
Immunocytochemistry/ Immunofluorescence: LIMPII/lpg85 Antibody [NB400-129] - Detection of LIMPII/lpg85 (Green) in Hela cells using NB400-129. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: LIMPII/lpg85 Antibody [NB400-129] - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

LIMPII/lpg85 Antibody Summary

Immunogen
A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]
Localization
Lysosome membrane; Multi-pass membrane protein.
Marker
Lysosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
Application Notes
This LIMPII/lpg85 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot, where a band is observed at ~85 kDa. Use in IHC-P was reported in scientific literature (PMID: 23863627). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Publications
Read Publications using
NB400-129 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for LIMPII/lpg85 Antibody

  • AMRF
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
  • CD36 antigen
  • CD36L2LIMP II
  • HLGP85
  • LGP85
  • LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
  • LIMPIICD36 antigen-like 2
  • lysosome membrane protein 2
  • Lysosome membrane protein II
  • Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
  • scavenger receptor class B, member 2
  • SR-BII

Background

LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIMPII/lpg85 Antibody (NB400-129)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Canine
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB400-129 Applications Species
Kondo H, Maksimova N, Otomo T et al. Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms. Hum. Mol. Genet. Dec 23 2016 [PMID: 28013294] (Human) Human
Mareninova OA, Sendler M, Malla SR et al. Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis. Cell Mol Gastroenterol Hepatol 2015 Nov 1 [PMID: 26693174] (Mouse) Mouse
Gurda BL, De Guilhem De Lataillade A, Bell P et al. Evaluation of AAV-mediated Gene Therapy for Central Nervous System Disease in Canine Mucopolysaccharidosis VII. Mol Ther 2016 Feb [PMID: 26447927]
Ikari A, Tonegawa C, Sanada A et al. Tight Junctional Localization of Claudin-16 is Regulated by Syntaxin 8 in Renal Tubular Epithelial Cells. J. Biol. Chem. 3/21/2014 [PMID: 24659781] (ICC/IF, Canine) ICC/IF Canine
Fruhwrth S, Pavelka M, Bittman R et al. High-density lipoprotein endocytosis in endothelial cells. World J Biol Chem 2013 Nov 26 [PMID: 24340136] (ICC/IF, Human) ICC/IF Human
Gaspar P, Kallemeijn WW, Strijland A et al. Action myoclonus-renal failure syndrome: diagnostic applications of activity-based probes and lipid analysis. J. Lipid Res. 2014 Jan 1 [PMID: 24212238] (WB, Human) WB Human
Haurigot V, Marco S, Ribera A et al. Whole body correction of mucopolysaccharidosis IIIA by intracerebrospinal fluid gene therapy. J Clin Invest 2013 Jul 1 [PMID: 23863627] (IHC-P, Mouse) IHC-P Mouse
Lee D, Desmond MJ, fraser SA et al. Expression of the Transmembrane Lysosomal Protein SCARB2/Limp-2 in Renin Secretory Granules Controls Renin Release. Nephron Exp Nephrol 2013 Apr 26 [PMID: 23635510]
Gleich K, Desmond MJ, Lee D et al. Abnormal Processing of Autophagosomes in Transformed B Lymphocytes from SCARB2-Deficient Subjects. Biores Open Access 2013 Feb [PMID: 23515316] (WB, Human) WB Human
Balreira A, Gaspar P, Caiola D et al. A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome. Hum Mol Genet;17(14):2238-43. 2008 Jul 15. [PMID: 18424452] (WB, Human) WB Human

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Product General Protocols

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ICC/IF Video Protocol

FAQs for LIMPII/lpg85 Antibody (NB400-129). (Showing 1 - 1 of 1 FAQs).

  1. Can you please inform me about the peptide used as immunogen? It is referred that is the a C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence, but the amino acid sequence used is not indicated.
    • NB400-129 was made to a sequence between amino acids 425-480.

Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SCARB2
Entrez