LIM kinase 2 Antibody

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Western Blot: LIM kinase 2 Antibody [NB100-91856] - Analysis of LIM kinase 2 (D499) antibody in extracts from NIH/3T3 treated with UV.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-91856
    • Availability
      Product Discontinued

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LIM kinase 2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 480-520 of Human LIMK2.
Specificity
LIM kinase 2 (D499) detects endogenous levels of LIMK2 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
LIMK2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of LIMK2 (D499) pAb in extracts from NIH/3T3 treated with UV.
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LIM kinase 2 Antibody

  • EC 2.7.11.1
  • LIM domain kinase 2
  • LIMK-2

Background

Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, incuding LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and Cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morpho-genesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and it is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LIMK2
Entrez