Lightning-Link Streptavidin Antibody Labeling Kit


Lightning-Link Streptavidin Antibody Labeling Kit [708-0010] - treptavidin-HRP prepared using Lightning-Link Streptavidin.

Product Details

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

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Lightning-Link Streptavidin Antibody Labeling Kit Summary

Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

Our streptavidin antibody labeling kit enables the direct conjugation of streptavidin to any biomolecule with an available amine group. The researcher simply pipettes their antibody or other biomolecule into the vial of Lightning-Link Streptavidin and incubates for 3 hours.

Key Features
  • 30 seconds hands-on time
  • No separation steps
  • No losses
  • Scalable technology
  • Quick and easy-to-use
  • Requires only 30 seconds hands-on time
  • No separation steps involved so recover 100% of you antibody or protein
  • Label from as little as 10ug to a gram or more!

Streptavidin is a 53kDa protein purified from Streptomyces avidinii, which has widespread applications due to its very high affinity to the vitamin biotin. Streptavidin is a tetrameric molecule, composed of 4 13kDa monomers, each of which can bind a molecule of biotin. The Lightning-Link® Streptavidin kit is unique in the sense that it is the only DIY streptavidin labeling kit available on the market.

Like our biotinylation kits, Lightning-Link® Streptavidin has been optimised to create high quality conjugates and minimise assay background. There is also no need for a wash or desalting step. The way in which the kits are optimised means the antibody or protein of interest will be labeled with up to 3 streptavidin molecules each, maximising the opportunity for biotin to bind and create a biotin streptavidin complex.
Kit Type
Antibody Labeling Kit

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.


Reviewed Applications
Read 2 Reviews rated 4
708-0010 in the following application:

Read Publications using 708-0010.

Kit Components

  1. Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size)
  2. 1 vial of LL-AP Modifier reagent
  3. 1 vial of LL-AP Quencher reagent


Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Fluorescent Labels Poster
Antibody Labeling Guide
Antibody Purification Guide

Lightning Link® is a registered trademark of Innova Biosciences.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lightning-Link Streptavidin Kit (708-0010)(17)

We have publications tested in 4 applications: ELISA, FLOW, IA, WB.

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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using 708-0010 Applications Species
Mann JF, Tregoning JS, Aldon Y et al. CD71 targeting boosts immunogenicity of sublingually delivered influenza haemagglutinin antigen and protects against viral challenge in mice J Control Release 2016 Jun 28 [PMID:27094605] (WB) WB
Madritsch C, Eckl-Dorna J, Blatt K et al. Antibody conjugates bispecific for intercellular adhesion molecule 1 and allergen prevent migration of allergens through respiratory epithelial cell layers. J Allergy Clin Immunol. 2015 [PMID:25769914] (ELISA, FLOW) ELISA, FLOW
Mallen M, Diaz-Gonzalez M, Bonilla D et al. Reusable conductimetric array of interdigitated microelectrodes for thereadout of low-density microarrays. Anal Chim Acta. 2014 [PMID:24890693] (IA) IA
Wienken U, Gaub HE. Stamping Vital Cells-a Force-Based Ligand Receptor Assay. Biophys J. 2013 [PMID:24359740]
Halpern MD, Jain S, Jewett MW. Enhanced detection of host response antibodies to Borrelia burgdorferi using immuno-PCR. Clin Vaccine Immunol 2013 [PMID:23302740] (IA) IA
Taylor RM, Sillerud LO. Paclitaxel-loaded iron platinum stealth immunomicelles are potent MRI imaging agents that prevent prostate cancer growth in a PSMA-dependent manner International Journal of Nanomedicine 2012 [PMID:22915856]
Lee H, Haque S, Nieto J et al. A p53 axis regulates B cell receptor-triggered, innate immune system-driven B cell clonal expansion. J Immunol. 2012 Jun 15 [PMID:22611237]
Yomogida K, Chou Y, Pang J et al. Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression Cyokine 2012 [PMID:22410319]
Kreuz G, Zagon J, Broll H et al. Immunological detection of osteocalcin in meat and bone meal: a novel heat stable marker for the investigation of illegal feed adulteration Food Additives & Contaminants: Part A 2011 [PMID:22300169] (IA) IA
Yoo SY, Oh JW, Lee SW. Phage-Chips for Novel Optically Readable Tissue Engineering Assays Langmuir 2011 [PMID:22149649]
Show All 17 Publications.

Reviews for Lightning-Link Streptavidin Kit (708-0010) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.
We have 2 review tested in 1 application: ELISA.

Reviews using 708-0010:
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Images Ratings Applications Species Date Details
reviewed by:
ELISA Human 05/03/2016


Sample TestedRANTES protein


Blocking Details1X PBS, 1% BSA, 1h, room temperature


Detection NotesPlate reader using TMB/H2SO4 at 450 nm absorption, 3 minutes, using 0 and 500 ng/mL
reviewed by:
ELISA Other 03/19/2009


Sample Testedtest of idea on solid surface
CommentsCan you monitor to see conjugation efficiency? I did conjugation rxn of Ab with your streptavidin conjugation reagent and then it was used to conjugate to biotinylated oligo. Absorbance measurement (A260 A280) can be used to see final conjugation efficiency between Ab-Streptavidin:Biotin-oligo following removing unreacted biotin-oligo.

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: 1% BSA and other components, Dilution Ratio: 1/100, Incubation Time: 2 hours, Incubation Temp: 25 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti Rb Ab conj using Lightning Link Streptavidin Conjugation Kit, Secondary Ab Dilution: 1:100
Secondary Manufacturer Cat#V-708-0010


CommentsCan you monitor to see conjugation efficiency? I did conjugation rxn of Ab with your streptavidin conjugation reagent and then it was used to conjugate to biotinylated oligo. Absorbance measurement (A260 A280) can be used to see final conjugation efficiency between Ab-Streptavidin:Biotin-oligo following removing unreacted biotin-oligo.

Product General Protocols

View specific protocols for Lightning-Link Streptavidin Kit (708-0010): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Lightning-Link Streptavidin Kit (708-0010). (Showing 1 - 10 of 11 FAQs).

  1. Can I label a peptide/protein/antibody other than IgG?
    • Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would recommend you adjust the amount of material you add to the Lighting-Link® vial to allow for molecular weight difference. This should be done without changing the volume added to the vial, as this could affect the conjugation efficiency. As a rough guideline, we would recommend changing the amount of material proportionally to the size difference with IgGs. An average IgG is about 160kD, therefore for a target that is half the size of an antibody (about 80kD), add half as much to the vial. Please note this is only a guideline and the best amount for your assay should be determined experimentally; our 3x10ug kits enable you to do this using small amounts of material and therefore at a low cost.
  2. Do I need a wash or desalt step?
    • One of the advantages of the Lightning-Link® technology compared to traditional labeling methods is that conjugate purification is not required. The Lightning-Link® reactions are highly efficient, and the kits have been optimized so that, provided the protocols are followed, there should be only a very low amount of free label left at the end of the conjugation. Any remaining free label would have its reactive 'Lightning-Link®' groups blocked by the Quencher provided in the kit, and would then be washed away during the relevant wash step of your application.
  3. Do my antibody and buffer fit the requirements?
    • Your antibody should be purified (affinity purification is preferred), as other molecules with free amine groups will interfere with the reaction, resulting in a poor quality conjugate. A suitable method of purification should be used (such as affinity or Protein A/G). Please note that 0.2/0.22 um filtration is a method of sterilization, not purification.Your biomolecule should also be in a suitable, 10-50mM amine free buffer (e.g. MES, MOPS, HEPES, PBS), pH range 6.5 to 8.5 and not in any of the following: ascites fluid, serum or tissue culture supernatant. It should not contain any additives such as Azide, BSA, Tris or Glycine at a concentration of >0.1%.The amount of antibody (IgG) you should add to the Lightning-Link® vial usually corresponds to the kit size your purchased (for example: a 3x100ug kit enable you to label 3 lots of 100ug antibody) and the volume added should also match (eg: 100ul), meaning the ideal concentration is of 1mg/ml. For other biomolecule, the amount added should be adjusted by changing your concentration (see above).NOTE: The amount and volume of antibody recommended above are for all Lightning-Link• kits offered by Innova Biosciences, with the exception of RPE and PE tandem dyes. For more details on the recommendations specific to these dyes, please consult the protocols available on each product page.
  4. How many labels will bind to my biomolecule?
    • It is difficult to give a precise answer to this as the result will vary from antibody to antibody (or other protein). Traditional labelling techniques often have F/P ratios in the range of 4-7:1, and our comparative data for staining with antibodies labelled with Lightning Link shows similar staining intensity. The ratio of dye to antibody is only ever an average for the population of labelled molecules, as individual molecules may have different amount of dye incorporated into them.
  5. How stable will my new conjugate be?
    • The Lightning-Link® chemistry joins the label to the antibody via a uni directional stable, covalent bond. The stability of your conjugate will therefore be dependent on your antibody and label of choice. In our in-house studies, conjugates made with our Lightning-Link® kits were fully active after more than 18 months' storage, undiluted, at 4 degrees C. Conjugates stored in 50% glycerol at -20 degrees C were found to be even more stable (several years). Please see below for advice on storage conditions. Please note that tandem dye stability is lower than the other dyes'. Tandem conjugates are fully stable for about 3 months at 4 degrees C.
  6. What are the best storage conditions for my new conjugate?
    • TA new conjugate can be stored for 12-18 months at 4 degrees C as long as the antibody will tolerate storage at 4 degrees C. As the bond between the antibody and dye is covalent and very stable, it should not degrade, therefore at 4 degrees C no additional preservatives are needed. The antibody is usually the least stable component of the conjugate so please check the antibody datasheet for storage recommendations. However, all conjugates can be stored in 50% glycerol at -20 degrees C which allows them to remain stable for 2 years. Please bear in mind that APC and RPE conjugates should never be stored at -20 degrees C on their own without glycerol. The dilution factor also has an effect. Storing the conjugate undiluted is recommended if possible; however, for HRP conjugates, if you wish to store at working concentrations (e.g. 1/10,000), this can be done using our LifeXtend HRP Conjugate Stabilizer/Diluent. Please note that the preservative sodium azide will inhibit HRP. We suggest that the optimal storage conditions for any conjugate are determined experimentally, using small aliquots of the conjugate.
  7. What can I do if my antibody formulation does not fit the requirements?
    • If your antibody buffer is not compatible with our kits, we have developed an AbSelect™ purification kit range that allows you to quickly and simply purify your antibody and is fully compatible with the Lightning-Link® kits.The appropriate kit to use depends on your particular sample (species, buffer, contaminants, volume,...). We have designed a handy flow chart on the AbSelect™ webpage to help you select a kit, but feel free to contact us if you require further guidance. Please consult the kit protocols to see the antibody amount/volume suitable for each kit.If your antibody is already purified but its concentration is too low, you can concentrate it by using our Antibody Concentration and Clean Up Kit. This kit can also be used to remove low molecular weight contaminants such as azide, tris or glycine by carrying out a buffer exchange into the buffer supplied in the kit, which is fully compatible with Lightning-Link®.If your antibody contains BSA, you can now use our BSA removal kit to purify your antibody in a few simple steps. Please note this kit will also enable you to concentrate your antibody.NB: All the AbSelect kits will ONLY work with antibodies. They will not purify other molecules. The only exception is the concentration and clean up kit (861-0010), which will work with other molecules greater than 10kD.
  8. What is the difference between Lightning-Link® and Lightning-Link® Rapid?
    • The benefit of the Rapid kits is that the conjugates can be generated faster because the conjugation and quenching incubation times are shorter. The antibody/protein/peptide considerations are exactly the same, as is the high quality of the final conjugate. DyLight® dyes are only available in the Rapid format.
  9. I have just ordered 708-0010 and I would like to know if you have any literature available?
    • If you go to the Images, Reviews & Publications tab of the product datasheet you can view the publications available. Also, if you go to the Protocols & FAQs tab of the product datasheet you can view protocols for this product.
  10. How does this kit conjugate antibodies to streptavidin? amine-thiol reaction or another one??
    • This kit works by labeling the free amine groups.
  11. Show All 11 FAQs.

Additional Lightning-Link Streptavidin Products

Lightning-Link Streptavidin 708-0010

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