Lightning-Link PerCP-Cy5.5 Antibody Labeling Kit


Lightning-Link PerCP-Cy5.5 Antibody Labeling Kit [763-0030] - Flow Cytometry: Lightning-Link PerCP-Cy5.5 Antibody Labeling Kit [763-0005] - Mouse anti-human CD3 was conjugated with PerCP/Cy5.5 using Lightning-Link® more
Lightning-Link PerCP-Cy5.5 Antibody Labeling Kit [763-0030]

Product Details

PerCP/Cy5.5 (A=488, E=695)
Kit Type
Antibody Labeling Kit

Order Details

Lightning-Link PerCP-Cy5.5 Antibody Labeling Kit Summary

PerCP/Cy5.5 is a tandem conjugate. The PerCP is excited at 482nm and functions as an energy donor for the Cy5.5. Energy is transferred from the PerCP to the Cy5.5 via energy resonance transfer. The Cy5.5 emits the energy received from the PerCP in the form of long wavelength light at 700nm.
Kit Type
Antibody Labeling Kit

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PerCP/Cy5.5 (A=488, E=695)


Application Notes
PerCP/Cy5.5 is a tandem conjugate. In our Lightning-Link PerCP/Cy5.5 the best result is obtained when the tandem is excited at 488nm, wavelength at which the PerCP has its maximum absorption. The excited PerCP functions as an energy donor, where the energy is transferred via FRET (Fluorescence Resonance Energy Transfer) to the Cy5.5. The Cy5.5 emits then the energy received from the PE in the form of long wavelength light at 700nm. Use in FLOW cytometry reported in scientific literature ( PMID 26483406)
Read Publications using 763-0030.

Kit Components

  1. Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size)
  2. 1 vial of LL Modifier reagent
  3. 1 vial of LL Quencher FD reagent


Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Fluorescent Labels Poster
Antibody Labeling Guide
Antibody Purification Guide

Lightning Link® is a registered trademark of Innova Biosciences.


Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.

The Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.

The technology is fully scalable (ug to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lightning-Link PerCP-Cy5.5 Kit (763-0030)(11)

We have publications tested in 1 application: FLOW.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 763-0030 Applications Species
Okagawa T, Konnai S, Deringer JR et al. Cooperation of PD-1 and LAG-3 contributes to T-cell exhaustion in Anaplasma marginale-infected cattle Infect Immun. 2016 Jul 18 [PMID:27430272] (FLOW) FLOW
Vremec D. The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and Their In Vitro Equivalents Methods Mol Biol. 2016 [PMID:27142009] (FLOW) FLOW
Riley SP, Fish AI, Garza DA et al. Non-selective Persistence of a Rickettsia conoriiExtrachromosomal Plasmid During Mammalian Infection Infect Immun. 2016 Jan 11 [PMID:26755154] (FLOW) FLOW
Dooley J, Garcia-Perez JE, Sreenivasan J et al. The microRNA-29 Family Dictates the Balance Between Homeostatic and Pathological Glucose Handling in Diabetes and Obesity Diabetes 2016 Jan [PMID:26696639] (FLOW) FLOW
Okagawa T, Konnai S, Nishimori A et al. Bovine immunoinhibitory receptors contribute to the suppression of Mycobacterium avium subsp.paratuberculosis-specific T-cell responses Infect Immun. 2015 Oct 19 [PMID:26483406] (FLOW) FLOW
Kitazawa Y, Ueta H, Hunig T et al. A novel multicolor immunostaining method using ethynyl deoxyuridine for analysis of in situ immunoproliferative response Histochem Cell Biol. 2015 Sep [PMID:25976155] (FLOW) FLOW
Slota C, Shi A, Chen G et al. Norepinephrine preferentially modulates memory CD8 T cell function inducing inflammatory cytokine production and reducing proliferation in response to activation. Brain Behav Immun. 2015 [PMID:25653192] (FLOW) FLOW
Goode D, Truong R, Villegas G et al. Driven Increase in the Expression of a4Beta7 Correlates with Increased Susceptibility to Vaginal SHIVSF162P3 Infection PLoS Pathog 2014 Dec 18 [PMID:25521298] (FLOW) FLOW
Robinson AP, Rodgers JM, Goings GE, Miller SD. Characterization of Oligodendroglial Populations in Mouse Demyelinating Disease Using Flow Cytometry: Clues for MS Pathogenesis. PLoS One 2014 [PMID:25247590] (FLOW) FLOW
Ikebuchi R, Konnai S, Okagawa T et al. Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro. Vet Res 2013 [PMID:23876077] (FLOW) FLOW
Show All 11 Publications.

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FAQs for Lightning-Link PerCP-Cy5.5 Kit (763-0030). (Showing 1 - 8 of 8 FAQs).

  1. Can I label a peptide/protein/antibody other than IgG?
    • Yes. The Lightning-Link® technology works by targeting free amine groups on your target, meaning it can be used to label most biomolecules.As the protocols provided were optimized for labeling IgGs, we would recommend you adjust the amount of material you add to the Lighting-Link® vial to allow for molecular weight difference. This should be done without changing the volume added to the vial, as this could affect the conjugation efficiency. As a rough guideline, we would recommend changing the amount of material proportionally to the size difference with IgGs. An average IgG is about 160kD, therefore for a target that is half the size of an antibody (about 80kD), add half as much to the vial. Please note this is only a guideline and the best amount for your assay should be determined experimentally; our 3x10ug kits enable you to do this using small amounts of material and therefore at a low cost.
  2. Do I need a wash or desalt step?
    • One of the advantages of the Lightning-Link® technology compared to traditional labeling methods is that conjugate purification is not required. The Lightning-Link® reactions are highly efficient, and the kits have been optimized so that, provided the protocols are followed, there should be only a very low amount of free label left at the end of the conjugation. Any remaining free label would have its reactive 'Lightning-Link®' groups blocked by the Quencher provided in the kit, and would then be washed away during the relevant wash step of your application.
  3. Do my antibody and buffer fit the requirements?
    • Your antibody should be purified (affinity purification is preferred), as other molecules with free amine groups will interfere with the reaction, resulting in a poor quality conjugate. A suitable method of purification should be used (such as affinity or Protein A/G). Please note that 0.2/0.22 um filtration is a method of sterilization, not purification.Your biomolecule should also be in a suitable, 10-50mM amine free buffer (e.g. MES, MOPS, HEPES, PBS), pH range 6.5 to 8.5 and not in any of the following: ascites fluid, serum or tissue culture supernatant. It should not contain any additives such as Azide, BSA, Tris or Glycine at a concentration of >0.1%.The amount of antibody (IgG) you should add to the Lightning-Link® vial usually corresponds to the kit size your purchased (for example: a 3x100ug kit enable you to label 3 lots of 100ug antibody) and the volume added should also match (eg: 100ul), meaning the ideal concentration is of 1mg/ml. For other biomolecule, the amount added should be adjusted by changing your concentration (see above).NOTE: The amount and volume of antibody recommended above are for all Lightning-Link• kits offered by Innova Biosciences, with the exception of RPE and PE tandem dyes. For more details on the recommendations specific to these dyes, please consult the protocols available on each product page.
  4. How many labels will bind to my biomolecule?
    • It is difficult to give a precise answer to this as the result will vary from antibody to antibody (or other protein). Traditional labelling techniques often have F/P ratios in the range of 4-7:1, and our comparative data for staining with antibodies labelled with Lightning Link shows similar staining intensity. The ratio of dye to antibody is only ever an average for the population of labelled molecules, as individual molecules may have different amount of dye incorporated into them.
  5. How stable will my new conjugate be?
    • The Lightning-Link® chemistry joins the label to the antibody via a uni directional stable, covalent bond. The stability of your conjugate will therefore be dependent on your antibody and label of choice. In our in-house studies, conjugates made with our Lightning-Link® kits were fully active after more than 18 months' storage, undiluted, at 4 degrees C. Conjugates stored in 50% glycerol at -20 degrees C were found to be even more stable (several years). Please see below for advice on storage conditions. Please note that tandem dye stability is lower than the other dyes'. Tandem conjugates are fully stable for about 3 months at 4 degrees C.
  6. What are the best storage conditions for my new conjugate?
    • TA new conjugate can be stored for 12-18 months at 4 degrees C as long as the antibody will tolerate storage at 4 degrees C. As the bond between the antibody and dye is covalent and very stable, it should not degrade, therefore at 4 degrees C no additional preservatives are needed. The antibody is usually the least stable component of the conjugate so please check the antibody datasheet for storage recommendations. However, all conjugates can be stored in 50% glycerol at -20 degrees C which allows them to remain stable for 2 years. Please bear in mind that APC and RPE conjugates should never be stored at -20 degrees C on their own without glycerol. The dilution factor also has an effect. Storing the conjugate undiluted is recommended if possible; however, for HRP conjugates, if you wish to store at working concentrations (e.g. 1/10,000), this can be done using our LifeXtend HRP Conjugate Stabilizer/Diluent. Please note that the preservative sodium azide will inhibit HRP. We suggest that the optimal storage conditions for any conjugate are determined experimentally, using small aliquots of the conjugate.
  7. What can I do if my antibody formulation does not fit the requirements?
    • If your antibody buffer is not compatible with our kits, we have developed an AbSelect™ purification kit range that allows you to quickly and simply purify your antibody and is fully compatible with the Lightning-Link® kits.The appropriate kit to use depends on your particular sample (species, buffer, contaminants, volume,...). We have designed a handy flow chart on the AbSelect™ webpage to help you select a kit, but feel free to contact us if you require further guidance. Please consult the kit protocols to see the antibody amount/volume suitable for each kit.If your antibody is already purified but its concentration is too low, you can concentrate it by using our Antibody Concentration and Clean Up Kit. This kit can also be used to remove low molecular weight contaminants such as azide, tris or glycine by carrying out a buffer exchange into the buffer supplied in the kit, which is fully compatible with Lightning-Link®.If your antibody contains BSA, you can now use our BSA removal kit to purify your antibody in a few simple steps. Please note this kit will also enable you to concentrate your antibody.NB: All the AbSelect kits will ONLY work with antibodies. They will not purify other molecules. The only exception is the concentration and clean up kit (861-0010), which will work with other molecules greater than 10kD.
  8. What is the difference between Lightning-Link® and Lightning-Link® Rapid?
    • The benefit of the Rapid kits is that the conjugates can be generated faster because the conjugation and quenching incubation times are shorter. The antibody/protein/peptide considerations are exactly the same, as is the high quality of the final conjugate. DyLight® dyes are only available in the Rapid format.

Additional Lightning-Link PerCP-Cy5.5 Products

Lightning-Link PerCP-Cy5.5 763-0030

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