Lightning-Link PE-Texas Red Antibody Labeling Kit Summary
Lightning-Link® is an innovative technology that enables direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
The procedure is
Easy to use
Requires 30 sec hands-on time
No spin or separation steps involved
The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates for either 3 hours or See Lighning-Link® Rapid for only 15 min incubation
Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.
Antibody Labeling Kit
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PE/Texas Red (A=566, E=616)
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
- Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size)
- 1 vial of LL Modifier reagent
- 1 vial of LL Quencher FD reagent
Lightning-Link is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.
Label - PE/Texas red is a tandem conjugate. The PE is excited at 488nm and functions as an energy donor for the Texas red. Energy is transferred from the PE to the Texas red via energy resonance transfer. The Texas red emits the energy received from the PE in the form of long wavelength light at 615nm.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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