Lightning-Link PE-Atto 594 Antibody Labeling Kit Summary
Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
Our PE/Atto594 antibody labeling kit enables the direct conjugation of the PE/Atto594 tandem label to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link label and incubates for 3 hours.
|Quick and easy to use||Save time, no special knowledge required|
|No separation steps||100% recovery - no antibody/protein loss|
|Can be used in a wide range of applications||Flexible|
|Freeze dried||Ships at ambient temperature, long shelf-life|
|Fully scalable (10 ug to 1 g or more)||Easy transfer from R&D to manufacturing|
|Stringently QC tested||Consistent high quality, excellent batch-to-batch reproducibility|
|Large number of labels available ||Experimental flexibility|
|Reliable: nearly 300 references||Successfully used in many fields of research|
The PE has three maximal absorbance values of 498, 544 and 566nm with the optimal depending on the application. The PE functions as an energy donor for the Atto594 fluorescent dye and transfers its energy via energy resonance transfer. The Atto594 emits the energy received from the PE in the form of long wavelength light at 632nm.Popular conjugates applications:
1. Flow Cytometry
2. Multi-Color Fluorescence (especially when in conjunction with singular dyes)
Antibody Labeling Kit
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
- 1 or 3 glass vial(s) of Lightning-Link mix
- 1 vial of LL-Quencher reagent
- 1 vial of LL-Modifier reagent
Lightning-Link is a one step antibody labeling kit, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.Label - PE/Atto594 is a completely new tandem conjugate. The PE is excited at 488nm and functions as an energy donor for the Atto594 fluorescent dye. Energy is transferred from the PE to the Atto594 dye via energy resonance transfer. The Atto594 emits the energy received from the PE in the form of long wavelength light at 627nm.Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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