Lightning-Link B-Phycoerythrin Antibody Labeling Kit

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Product Details

Summary
Format
B-Phycoerythrin (A=545;563, E=578)
Kit Type
Antibody Labeling Kit

Order Details

Lightning-Link B-Phycoerythrin Antibody Labeling Kit Summary

Description
Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

Our B-PE antibody labeling kit enables the direct conjugation of B-PE to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link R-PE and incubates for 3 hours.

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


BPE is a fluorescent protein from the phycobiliprotein family from the cyanbacteria and eukaryotic algae. It has a strong absorption peak at about 546 nm and has an emission value of 580 nm.

B-Phycoerythrin is often thought to be less "sticky" than R-Phycoerythrin therefore BPE conjugates tend to produce less background signal from non-specific binding in certain applications.

Popular conjugates applications:
1. Flow Cytometry
2. Immunofluorescence
Kit Type
Antibody Labeling Kit

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
B-Phycoerythrin
Format
B-Phycoerythrin (A=545;563, E=578)

Applications/Dilutions

Publications
Read Publications using 716-0005.

Kit Components

Components
  1. 1 or 3 glass vial(s) of Lightning-Link mix
  2. 1 vial of LL-Modifier reagent
  3. 1 vial of LL-Quencher reagent

Notes

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Fluorescent Labels Poster
Antibody Labeling Guide
Antibody Purification Guide

Lightning Link® is a registered trademark of Innova Biosciences.

Background

Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lightning-Link B-Phycoerythrin Kit (716-0005)(6)

We have publications tested in 1 application: FLOW.


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Product General Protocols

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Lightning-Link B-Phycoerythrin 716-0005

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