Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures. Features
30 seconds hands-on time
No separation steps
Easy to use antibody labeling kit
Antibody recovery is 100%
Applicable to Western Blotting, ELISA, Immuohistochemistry, Immunofluorescence, and FACS analysis
From 10ug up to 5mg
B-Phycoerythrin (B-PE) is a fluorescent protein from phycobiliprotein family. It has a primary absorption peak at 546 nm and a secondary peak at 563 nm. When excited at 546 nm it has a strong fluorescence emission with its maximum at 575 nm.
Antibody Labeling Kit
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
B-Phycoerythrin (A=545;563, E=578)
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Lightning Link® is a registered trademark of Innova Biosciences.
Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for Lightning-Link B-Phycoerythrin Kit (716-0005)(6)
We have publications tested in 1 application: FLOW.