LIF R alpha Antibody [Unconjugated]

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Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.3 ng/mL Recombinant Human LIF is neutralized (green ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LIF R alpha Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LIF R alpha
Gln45-Ser833
Accession # P42702
Specificity
Detects human LIF R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 7% cross-reactivity with recombinant mouse LIF R alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIFR
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 6-36 ug/mL
Publications
Read Publications using
AF-249-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF R alpha Antibody [Unconjugated]

  • CD118 antigen
  • CD118
  • FLJ98106
  • FLJ99923
  • leukemia inhibitory factor receptor alpha
  • leukemia inhibitory factor receptor
  • LIF R alpha
  • LIF R beta
  • LIF receptor
  • LIFR
  • LIF-R
  • LIFRa
  • SJS2
  • STWS
  • SWS

Background

The activities of the pleiotropic cytokine LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha  subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Besides LIF, the high-affinity heterodimeric LIF receptor complex has been shown to mediate the activities of oncostatin M (OSM), cardiotrophin-1 and ciliary neurotrophic factor (CNTF).

Human LIF R alpha  cDNA encodes a 1097 amino acid (aa) residue precursor type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. LIF R alpha is a member of the cytokine receptor family and has extensive homology to gp130. The extracellular domain of LIF R alpha  has two cytokine receptor domains and three fibronectin type III repeats. In mouse, mRNAs encoding a soluble LIF R alpha and lacking transmembrane and intracellular domains, have been isolated. Soluble LIF R alpha has been shown to bind LIF and has LIF antagonistic activity.

  1. Bazan, J.F. (1990) Proc. Natl. Acad. Sci. USA 87:6934.
  2. Gearing, D.P. (1994) Guidebook to Cytokines and Their Receptors, Academic Press, p130.
  3. Pennica D. et al. (1995) J. Biol. Chem. 270:10915.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIF R alpha Antibody (AF-249-NA)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Development, ICC, IHC Fresh/Frozen, IHC Paraffin-embedded, Neut, WB.


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ELISA Development
(1)
ICC
(1)
IHC Fresh/Frozen
(2)
IHC Paraffin-embedded
(2)
Neut
(3)
WB
(2)
All Applications
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Human
(10)
Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF-249-NA Applications Species
Salm F, Dimitrova V, von Bueren A, Cwiek P, Rehrauer H, Djonov V, Anderle P, Arcaro A The Phosphoinositide 3-Kinase p110alpha Isoform Regulates Leukemia Inhibitory Factor Receptor Expression via c-Myc and miR-125b to Promote Cell Proliferation in Medulloblastoma. PLoS ONE, 2015;10(4):e0123958. 2015 [PMID: 25915540] (Neut,, Human) Neut Human
Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor. J Biol Chem, 2012;287(39):32848-59. 2012 [PMID: 22829597] (ELISA Development,, Human) ELISA Development Human
Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A Disturbances in the LIF pathway in the endometrium among women with unexplained infertility. Fertil. Steril., 2009;91(6):2602-10. 2009 [PMID: 18684446] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells. Fertil. Steril., 2006;86(4):1193-209. 2006 [PMID: 16949591] (ICC,, Human) ICC Human
Pan W, Cain C, Yu Y, Kastin AJ Receptor-mediated transport of LIF across blood-spinal cord barrier is upregulated after spinal cord injury. J. Neuroimmunol., 2006;174(1):119-25. 2006 [PMID: 16563523] (Neut,, Mouse) Neut Mouse
Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression. Cancer Res., 2005;65(19):8896-904. 2005 [PMID: 16204061] (Neut,, Human) Neut Human
He W, Gong K, Smith DK, Ip NY The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett., 2005;579(20):4317-23. 2005 [PMID: 16051226] (WB,, Human) WB Human
Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Mol. Hum. Reprod., 2004;10(5):313-9. 2004 [PMID: 15044601] (IHC Fresh/Frozen,, Human) IHC Fresh/Frozen Human
Malm C, Sjodin TL, Sjoberg B, Lenkei R, Renstrom P, Lundberg IE, Ekblom B Leukocytes, cytokines, growth factors and hormones in human skeletal muscle and blood after uphill or downhill running. J. Physiol. (Lond.), 2004;556(0):983-1000. 2004 [PMID: 14766942] (IHC Fresh/Frozen,, Human) IHC Fresh/Frozen Human
Ben-Shlomo A, Miklovsky I, Ren SG, Yong WH, Heaney AP, Culler MD, Melmed S Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells. J. Clin. Endocrinol. Metab., 2003;88(2):858-63. 2003 [PMID: 12574225] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol LIFR
Uniprot