Lgr4/GPR48 Antibody (2D1)

Images

 
Western Blot: GPR48 Antibody (2D1) [NBP2-00974] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPR48 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Flow Cytometry: GPR48 Antibody (2D1) [NBP2-00974] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GPR48 antibody, and then analyzed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
2D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lgr4/GPR48 Antibody (2D1) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 230-540 of human LGR4 (NP_060960 ) produced in HEK293T Cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LGR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lgr4/GPR48 Antibody (2D1)

  • GPR48
  • GPR48G protein-coupled receptor 48
  • G-protein coupled receptor 48
  • leucine-rich repeat containing G protein-coupled receptor 4
  • Lgr4

Background

G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Lgr4/GPR48 Antibody (NBP2-00974). (Showing 1 - 2 of 2 FAQ).

  1. Is ab119314 from Abcam the same as NBP2-00974 from Novus? Because of the amount difference of supplemented information, Example Tested applications (Flow Cyt ) I have doubt, so I need your confirmation to show that these two are identical products.
    • Both antibodies have the same immunogen (human recombinant protein fragment corresponding to amino acids 230-540 of human LGR4 [NP_060960] produced in HEK293T Ce) and clone number (2D1). I have just confirmed that NBP2-00974 is useful for Flow Cytometery in addition to WB application. This antibody works well at following dilutions: Flow Cytometry 1:100, Western Blot 1:500.
  2. May we ask if the product is powder or liquid?
    • This antibody comes in a liquid format.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol LGR4
Entrez
Uniprot