Recombinant Leptin/OB Protein

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43172
    • Availability
      Product Discontinued

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Recombinant Leptin/OB Protein Summary

Description
A partial protein corresponding to LEP.
Preparation
Method
E. Coli.
Details of Functionality
This reagent has been tested in bioassays using the human OB-R transfected mouse BaF/3 cell line. The ED50 measured in the human OB-R transfected mouse BaF/3 cell proliferation assay is typically 1.36 ng/ml, corresponding to a specific activity of approximately 7 x10^5Units/mg.
Protein/Peptide Type
Partial Recombinant Protein
Gene
LEP
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 um filtered.
Preservative
0.09% Sodium Azide

Notes

Purity: Greater than 95%, as determined by SDS-PAGE

Alternate Names for Recombinant Leptin/OB Protein

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin is a protein made by adipose cells. Leptin inhibits food consumption and stimulates energy expenditure. Leptin has been shown to reduce body weight and plasma glucose levels. Leptin plays a critical role in the obesity of mice and humans. It is also reported that leptin functions as a regulatory cytokine in hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant human leptin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of about 16 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol LEP
Entrez