LDL R Antibody

Images

 
Western Blot: LDL R Antibody [NBP1-06709] - Western Blot on HepG2 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: LDL R Antibody [NBP1-06709] - LDL receptor antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry-Paraffin: LDL R Antibody [NBP1-06709] - LDL Receptor was detected in immersion fixed paraffin-embedded sections of human liver cancer using rabbit anti-human antibody (Catalog # NBP1-06709) at ...read more
Simple Western: LDL R Antibody [NBP1-06709] - Simple Western lane view shows a specific band for LDL R in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.41 mg/ml

LDL R Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human LDL Receptor protein (within residues 500-550). [Swiss-Prot# P01130]
Localization
Membrane; Single-pass type I membrane protein. Clathrin-coated pit.
Specificity
This is specific for both the unglycosylated and glycosylated forms of the LDL Receptor.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDLR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:1000-1:10000
  • Immunohistochemistry 1:1000 - 1:3000
  • Immunohistochemistry-Paraffin 1:1000 - 1:3000
Application Notes
This LDL Receptor antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where bands are seen ~95 kDa and ~160 kDa representing the unglycosylated and glycosylated forms of the LDL receptor, respectively.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
LDL R Lysate (NBL1-12475)
Control Peptide
LDL R Peptide (NBP1-06709PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-06709 in the following applications:

Publications
Read Publications using
NBP1-06709 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Human and Mouse. Predicted to react with monkey based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.41 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LDL R Antibody

  • FH
  • FHC
  • LDL R
  • LDL receptor
  • LDLCQ2
  • LDLR
  • low density lipoprotein receptor
  • low-density lipoprotein receptor class A domain-containing protein 3
  • low-density lipoprotein receptor

Background

LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LDL R Antibody (NBP1-06709)(3)

Review for LDL R Antibody (NBP1-06709) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-06709:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot LDL R NBP1-06709
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reviewed by:
Dina Schneider
WB Mouse 12/08/2016
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Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesMouse

Product General Protocols

View specific protocols for LDL R Antibody (NBP1-06709): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LDL R Antibody (NBP1-06709) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-06709AF405
Alexa Fluor 488 Labeled NBP1-06709AF488
Alexa Fluor 647 Labeled NBP1-06709AF647
Alexa Fluor 700 Labeled NBP1-06709AF700
Biotin Labeled NBP1-06709B
DyLight 405 Labeled NBP1-06709V
DyLight 488 Labeled NBP1-06709G
DyLight 550 Labeled NBP1-06709R
DyLight 650 Labeled NBP1-06709C
FITC Labeled NBP1-06709F
HRP Labeled NBP1-06709H

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Bioinformatics Tool for LDL R Antibody (NBP1-06709)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Dina Schneider
12/08/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol LDLR
Entrez