LDL R Antibody (C7)

Images

 
Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane ...read more
Immunocytochemistry/ Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.
Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and ...read more
Immunocytochemistry/ Immunofluorescence: LDL R Antibody (C7) [NBP1-78159] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).

Product Details

Summary
Reactivity Hu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, IP, RIA
Clone
C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

LDL R Antibody (C7) Summary

Immunogen
Partially purified bovine LDL receptor [Swiss-Prot# P01131]
Localization
Cell membrane/cell surface, endomembrane system, endosomes, lysosome, clathrin-coated pit, and Golgi apparatus
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LDLR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Electron Microscopy
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Radioimmunoassay
Application Notes
In WB assay, specific bands can be seen around 160 kDa (mature form) and 120 kDa (precursor) molecular weight positions. Use in FLOW cytometry reported in scientific literature ( PMID 10906332). Use in Electron Microscopy and Immunohistochemistry frozen reported in scientific literature (PMID 11839845). Use in In vitro reported in scientific literature (PMID 9642270).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-78159 in the following applications:

Publications
Read Publications using
NBP1-78159 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for LDL R Antibody (C7)

  • FH
  • FHC
  • LDL R
  • LDL receptor
  • LDLCQ2
  • LDLR
  • low density lipoprotein receptor
  • low-density lipoprotein receptor class A domain-containing protein 3
  • low-density lipoprotein receptor

Background

LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-527
Species: Hu, Mu
Applications: WB
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP2-31364
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91996
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP2-52506
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
AF4779
Species: Hu
Applications: WB, IHC
NBP2-37477
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NBP1-54446
Species: Hu, Ch
Applications: WB, ELISA, GS, ICC/IF, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-33002
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-33560
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NLS6792
Species: Hu, Mu, Po, Bt, Bv, Ca, Eq, Ha, Mk, Pm, Rb
Applications: IHC-P
NBP2-52979
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-78162
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha
Applications: WB, Simple Western
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P

Publications for LDL R Antibody (NBP1-78159)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 9 applications: ELISA, EM, FLOW, ICC/IF, IHC-Fr, IP, In vitro, RIA, WB.


Filter By Application
ELISA
(1)
EM
(1)
FLOW
(2)
ICC/IF
(4)
IHC-Fr
(1)
IP
(3)
In vitro
(1)
RIA
(2)
WB
(4)
All Applications
Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-78159 Applications Species
Chen Hc, Chen Py, Wu Mj et al. Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells. PLoS ONE Sep 13 2016 [PMID: 27617748] (WB) WB
Granato M, Zompetta C, Vescarelli E et al. HCV derived from sera of HCV-infected patients induces pro-fibrotic effects in human primary fibroblasts by activating GLI2. Sci Rep. Aug 1 2016 [PMID: 27476557] (WB, Human) WB Human
Pena F, Jansens A, van Zadelhoff G, Braakman I. Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum. J Biol Chem. 2010 Mar 19 [PMID: 20089850]
Roosendaal SD, Kerver J, Schipper M et al. The complex of the insect LDL receptor homolog, lipophorin receptor, LpR, and its lipoprotein ligand does not dissociate under endosomal conditions. FEBS J. 2008 Apr [PMID: 18331356] (FLOW) FLOW
Blasiole DA, Oler AT, Attie AD. Regulation of ApoB secretion by the low density lipoprotein receptor requires exit from the endoplasmic reticulum and interaction with ApoE or ApoB. J Biol Chem. 2008 Apr 25 [PMID: 18272520] (ICC/IF) ICC/IF
Nguyen AT, Hirama T, Chauhan V et al. Binding characteristics of a panel of monoclonal antibodies against the ligand binding domain of the human LDLr. J Lipid Res. 2006 Jul [PMID: 16601300]
Maxwell KN, Fisher EA, Breslow JL. Overexpression of PCSK9 accelerates the degradation of the LDLR in a post-endoplasmic reticulum compartment. Proc Natl Acad Sci U S A. 102(6):2069-74. 2005 Feb 8. [PMID: 15677715] (IP, Human) IP Human
Gillian-Daniel DL, Bates PW, Tebon A, Attie AD. Endoplasmic reticulum localization of the low density lipoprotein receptor mediates presecretory degradation of apolipoprotein B. Proc Natl Acad Sci U S A. 99(7):4337-42. 2002 Apr 2. [PMID: 11904390] (ICC/IF, Human) ICC/IF Human
Jaworska-Wilczynska M, Wilczynski GM, Engel WK et al. Three lipoprotein receptors and cholesterol in inclusion-body myositis muscle. Neurology. 2002 Feb 12 [PMID: 11839845] (IHC-Fr, EM, Human) IHC-Fr, EM Human
Jorgensen MM1, Jensen ON, Holst HU et al. Grp78 is involved in retention of mutant low density lipoprotein receptor protein in the endoplasmic reticulum. J Biol Chem. 2000 Oct 27 [PMID: 10906332] (IP, FLOW, Human) IP, FLOW Human
Show All 16 Publications.

Review for LDL R Antibody (NBP1-78159) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-78159:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry LDL R NBP1-78159
Enlarge
reviewed by:
Kwonil Jung
ICC Other 12/03/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedA porcine intestinal epithelial cell line, named as IPEC-J2
SpeciesOther

Blocking

Blocking Details1x Powerblocking solution in distilled water (Biogenex)

Primary Anitbody

Dilution Ratio1:200 in PBS overnight

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, Alexa Fluor 488
Secondary Manufacturer Cat#A11017 Invitrogen
Secondary Concentration1:300 in PBS

Details

Fixation Details75% Acetone/25% ethanol used for fixation, antigen retrieval, and permeabilization for 30 min at room tem
Wash DescriptionVigorous wash with PBS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LDL R Antibody (NBP1-78159) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-78159AF405
Alexa Fluor 488 Labeled NBP1-78159AF488
Alexa Fluor 647 Labeled NBP1-78159AF647
Alexa Fluor 700 Labeled NBP1-78159AF700
Allophycocyanin Labeled NBP1-78159APC
Biotin Labeled NBP1-78159B
DyLight 350 Labeled NBP1-78159UV
DyLight 405 Labeled NBP1-78159V
DyLight 488 Labeled NBP1-78159G
DyLight 550 Labeled NBP1-78159R
DyLight 650 Labeled NBP1-78159C
DyLight 680 Labeled NBP1-78159FR
DyLight 755 Labeled NBP1-78159IR
FITC Labeled NBP1-78159F
HRP Labeled NBP1-78159H
PE Labeled NBP1-78159PE

Additional LDL R Products

Bioinformatics Tool for LDL R Antibody (NBP1-78159)

Discover related pathways, diseases and genes to LDL R Antibody (NBP1-78159). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LDL R Antibody (NBP1-78159)

Discover more about diseases related to LDL R Antibody (NBP1-78159).
 

Pathways for LDL R Antibody (NBP1-78159)

View related products by pathway.

PTMs for LDL R Antibody (NBP1-78159)

Learn more about PTMs related to LDL R Antibody (NBP1-78159).
 

Research Areas for LDL R Antibody (NBP1-78159)

Find related products by research area.

Blogs on LDL R

There are no specific blogs for LDL R, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Kwonil Jung
12/03/2013
Application: ICC
Species: Other

Bioinformatics

Gene Symbol LDLR
Entrez
Uniprot