LD78 beta Antibody

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Western Blot: LD78 beta Antibody [NBP1-42775] - Analysis of recombinant swine CCL3L1 at 7.8kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked ...read more

Product Details

Summary
Reactivity Po, BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

LD78 beta Antibody Summary

Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 70 amino acids of the mature swine CCL3L1 protein.
Specificity
This antibody is specific for swine CCL3L1 protein. A BLAST analysis was used to suggest cross-reactivity with CCL3L1 from bovine sources based on 100% identity with the immunizing sequence. Partial reactivity is expected against CCL3L1 from cat, dog, human, chimpanzee, baboon, bovine, and rat based on greater than 90% sequence homology. Cross-reactivity with CCL3L1 from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCL3L1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
Application Notes
This protein-A purified antibody has been tested for use in ELISA and western blotting.Expect a band approximately 7.8 kDa in size corresponding - swine CCL3L1 protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

swine and bovine

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.

Alternate Names for LD78 beta Antibody

  • C-C motif chemokine 3-like 1
  • CCL3L3
  • chemokine (C-C motif) ligand 3-like 1
  • D17S1718
  • G0/G1 switch regulatory protein 19-2
  • G0S19-2LD78
  • LD78BETA
  • LD78-beta(1-70)
  • MGC104178
  • MGC12815
  • MGC182017
  • MIP1AP
  • PAT 464.2
  • SCYA3L
  • SCYA3L1464.2
  • small inducible cytokine A3-like 1
  • Small-inducible cytokine A3-like 1
  • Tonsillar lymphocyte LD78 beta protein

Background

Chemokine (C-C motif) ligand 3-like 1 (CCL3L1), also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha; other synonyms: ld78beta, scya3l1, small inducible cytokine a3-like 1), is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. In humans, CCL3L1 is encoded by a variable copy-number gene. The CC family induces MCP1 and RANTES, and exhibits a variety of proinflammatory activities including chemotaxis, and functional and proliferative activation of leukocytes, lymphocytes, and macrophages. Its signal is transmitted through transmembrane receptors, CC chemokine receptors, CCR1, CCR3 and CCR5.
CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. CCL3L1 binds to several chemokine receptors. In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CCL3L1