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Immunogen affinity purified
This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. Use in Immunohistochemistry-Frozen sections reported in scientific literature (PMID: 20008275) In Western blot bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells.
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Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602). Canine reactivity reported in scientific literature (PMID: 25839646). The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus.
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Alternate Names for LC3B/MAP1LC3B Antibody [DyLight 550]
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
lc3b autophagy marker
MAP1 light chain 3-like protein 2
MAP1A/MAP1B LC3 B
microtubule associated protein 3 b
microtubule associated protein 3 beta
microtubule-associated protein 1 light chain 3 betaMAP1A/MAP1B light chain 3 B
LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
LC3B - a novel marker for autophagosome Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a... Read full blog post.