LC3A Antibody

Images

 
Western Blot: LC3/MAP1LC3A Antibody [H00084557-D01P] - Analysis of MAP1LC3A expression in transfected 293T cell line by MAP1LC3A polyclonal antibody.Lane 1: MAP1LC3A transfected lysate(13.31 KDa).Lane 2: Non-transfected ...read more

Product Details

Summary
Product Discontinued
View other related LC3A Primary Antibodies

Order Details


    • Catalog Number
      H00084557-D01P
    • Availability
      Product Discontinued

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LC3A Antibody Summary

Immunogen
MAP1LC3A (AAH15810.1, 1 a.a. ~ 121 a.a) full-length human protein.MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF
Marker
Autophagosome Marker
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1LC3A
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
This antibody is useful for Western Blot

Reactivity Notes

This product is reactive against Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LC3A Antibody

  • Apg8
  • APG8a
  • Apg8p3
  • ATG8E
  • Autophagy-related protein LC3 A
  • Autophagy-related ubiquitin-like modifier LC3 A
  • LC3
  • LC3A
  • lc3-i, ii
  • MAP1A/1B light chain 3 A
  • MAP1ALC3
  • MAP1ALC3MAP1A/MAP1B LC3 A
  • MAP1BLC3MAP1A/MAP1B light chain 3 A
  • MAP1LC3A
  • microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1
  • microtubule-associated proteins 1A/1B light chain 3
  • microtubule-associated proteins 1A/1B light chain 3A
  • MLP3A

Background

LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for LC3A Antibody (H00084557-D01P). (Showing 1 - 1 of 1 FAQ).

  1. May we ask if it is possible to perform IF to stain LC3-I and LC3-II separately with two different fluorescent colors?
    • Yes, it is possible to perform IF stain for LC3-I and LC3-II separately with two different fluorescent colors! You will have to use two different primary antibodies and in order to avoid any potential background/cross reactivity issues, I would suggest that you employ conjugated primary antibodies for the testing. 1. Our LC3I antibody (NBP1-78964) has been designed to specifically detect the cytosolic form of the LC3 protein which is actually LC3 I (Note: LC3-II binds to the autophagic membranes). 2. There is not even a single antibody to our knowledge that would exclusively detect the LC3 II form, and you would have to detect LC3II/ autophagic membranes form with an antibody which detects LC3 I/LC3II together. Therefore you may opt second antibody from one of the followings: LC3 Antibody (NB100-2220), LC3 Antibody (NB100-2331), LC3 Antibody (NBP1-19167). All of these mentioned catalog #s come with different options for their conjugated forms and you may select appropriate conjugated forms for performing the IF staining using our explained criteria.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP1LC3A
Entrez
Uniprot