LC3A Antibody (1D5)

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ELISA: LC3/MAP1LC3A Antibody (1D5) [H00084557-M07] - Detection limit for recombinant GST tagged MAP1LC3A is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00084557-M07
    • Availability
      Product Discontinued

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LC3A Antibody (1D5) Summary

Immunogen
MAP1LC3A (NP_115903, 1 a.a. ~ 103 a.a) partial recombinant protein with GST tag.MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQE
Marker
Autophagosome Marker
Specificity
MAP1LC3A (1D5)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAP1LC3A
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against recombinant protein on ELISA. GST alone used as a negative control. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publication using
H00084557-M07 in the following applications:

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LC3A Antibody (1D5)

  • Apg8
  • APG8a
  • Apg8p3
  • ATG8E
  • Autophagy-related protein LC3 A
  • Autophagy-related ubiquitin-like modifier LC3 A
  • LC3
  • LC3A
  • lc3-i, ii
  • MAP1A/1B light chain 3 A
  • MAP1ALC3
  • MAP1ALC3MAP1A/MAP1B LC3 A
  • MAP1BLC3MAP1A/MAP1B light chain 3 A
  • MAP1LC3A
  • microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1
  • microtubule-associated proteins 1A/1B light chain 3
  • microtubule-associated proteins 1A/1B light chain 3A
  • MLP3A

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00084557-M07
Species: Hu
Applications: ELISA, ICC/IF

Publications for LC3A Antibody (H00084557-M07)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for LC3A Antibody (H00084557-M07). (Showing 1 - 1 of 1 FAQ).

  1. May we ask if it is possible to perform IF to stain LC3-I and LC3-II separately with two different fluorescent colors?
    • Yes, it is possible to perform IF stain for LC3-I and LC3-II separately with two different fluorescent colors! You will have to use two different primary antibodies and in order to avoid any potential background/cross reactivity issues, I would suggest that you employ conjugated primary antibodies for the testing. 1. Our LC3I antibody (NBP1-78964) has been designed to specifically detect the cytosolic form of the LC3 protein which is actually LC3 I (Note: LC3-II binds to the autophagic membranes). 2. There is not even a single antibody to our knowledge that would exclusively detect the LC3 II form, and you would have to detect LC3II/ autophagic membranes form with an antibody which detects LC3 I/LC3II together. Therefore you may opt second antibody from one of the followings: LC3 Antibody (NB100-2220), LC3 Antibody (NB100-2331), LC3 Antibody (NBP1-19167). All of these mentioned catalog #s come with different options for their conjugated forms and you may select appropriate conjugated forms for performing the IF staining using our explained criteria.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP1LC3A
Entrez
OMIM
Uniprot