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Immunogen affinity purified
This LC3 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence (PMID 21545732), Immunoprecipitation and Immunohistochemistry paraffin embedded sections. By Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. In ICC/IF, cytoplasmic staining was observed in HeLa cells.Use in FLOW cytometry reported in scientific literature ( PMID 24419333)
Human, mouse, rat and Zebrafish.Fish reactivity reported in scientific literature (PMID: 25522711) Predicted to react with Xenopus and bovine based on 100% sequence homology. Canine reactivity reported in scientific literature (PMID: 26179070)
Alternate Names for LC3/MAP1LC3A Antibody [Biotin]
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
MAP1A/1B light chain 3 A
MAP1ALC3MAP1A/MAP1B LC3 A
MAP1BLC3MAP1A/MAP1B light chain 3 A
microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for LC3/MAP1LC3A Antibody (NB100-2331B). (Showing 1 - 1 of 1 FAQ).
May we ask if it is possible to perform IF to stain LC3-I and LC3-II separately with two different fluorescent colors?
Yes, it is possible to perform IF stain for LC3-I and LC3-II separately with two different fluorescent colors! You will have to use two different primary antibodies and in order to avoid any potential background/cross reactivity issues, I would suggest that you employ conjugated primary antibodies for the testing. 1. Our LC3I antibody (NBP1-78964) has been designed to specifically detect the cytosolic form of the LC3 protein which is actually LC3 I (Note: LC3-II binds to the autophagic membranes). 2. There is not even a single antibody to our knowledge that would exclusively detect the LC3 II form, and you would have to detect LC3II/ autophagic membranes form with an antibody which detects LC3 I/LC3II together. Therefore you may opt second antibody from one of the followings: LC3 Antibody (NB100-2220), LC3 Antibody (NB100-2331), LC3 Antibody (NBP1-19167). All of these mentioned catalog #s come with different options for their conjugated forms and you may select appropriate conjugated forms for performing the IF staining using our explained criteria.