Latent TGF-beta bp1 Antibody (35409) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: WB, AP
• Clone: 35409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Latent TGF-beta bp1 Antibody (35409) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Human platelet-derived large latent TGF-beta 1 complex
• Specificity: Detects human LTBP-1 from the large latent TGF-beta 1 complex.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTBP1
• Purity: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Latent TGF-beta bp1 Antibody (35409) [CoraFluor™ 1]

• Latent TGFbeta bp1
• Latent TGF-beta bp1
• latent transforming growth factor beta binding protein 1
• latent-transforming growth factor beta-binding protein 1
• LTBP1
• LTBP-1
• MGC163161
• TGF-beta1-BP-1
• Transforming growth factor beta-1-binding protein 1

Background

LTBP1, also known as latent-transforming growth factor beta-binding protein 1, consists of five isoforms of sizes 186.8 kDa, 152.9 kDa, 147.2 kDa, 186.9 kDa, and 153 kDa and is involved in directing and regulating the activity of TGFB1. Current research is being conducted on diseases and disorders such as cataracts, fibrosis, geleophysic dysplasia, macular degeneration, aortic aneurysm, malignant glioma, hepatitis, vaginitis, scleroderma, and nephropathy. The protein is involved in extracellular matrix organization, mitochondrial apoptosis, TGF-beta signaling, and GSK3 signaling pathways, where it interacts with FN1, FBN2, TGFB1, ITGB5, and ATN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LTBP1