LAT Antibody (LAT1111)

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-49144
    • Availability
      Product Discontinued

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LAT Antibody (LAT1111) Summary

Immunogen
This antibody was generated by immunizing host with Partial recombinant protein (cytoplasmic domain of human LAT)
Localization
Lipid rafts, potential type III transmembrane protein, juxtanuclear intracellular compartment
Specificity
This antibody has been tested to recognize phosphorylated and nonphosphorylated forms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAT
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this LAT antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human (does not cross-react with mouse).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for LAT Antibody (LAT1111)

  • 36 kDa phospho-tyrosine adapter protein
  • LAT
  • LAT1,36 kDa phospho-tyrosine adaptor protein
  • linker for activation of T cells
  • linker for activation of T-cells family member 1
  • p36-38
  • pp36

Background

LAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-gamma1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl. The LAT1111 monoclonal antibody recognizes the cytoplasmic domain of human LAT and has been shown to be useful for Western blotting and immunoprecipitation. The LAT1111 antibody does not cross-react with mouse LAT. Grb2, Gads, Grap, PLC-gamma1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, cbl

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Research Areas for LAT Antibody (NBP1-49144)

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Blogs on LAT.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

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Bioinformatics

Gene Symbol LAT
Entrez