LAT Antibody (LAT.10-17) [Allophycocyanin]

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Flow Cytometry: LAT Antibody (LAT.10-17) [Allophycocyanin] [NBP2-00290] - Surface staining of normal human peripheral blood cells with Anti-Human CD3 FITC followed by intracellular staining with Mouse IgG1 kappa ...read more

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP2-00290
    • Availability
      Product Discontinued

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LAT Antibody (LAT.10-17) [Allophycocyanin] Summary

Immunogen
The immunogen for this antibody is LAT.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LAT
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
LAT.10-17 (10-17) was tested by western blot and immunoprecipitation on lysates prepared from human peripheral blood cells, Jurkat cells, and C57BL/6 splenocytes. This antibody can be used at 5 ug/mL. This LAT.10-17 (10-17) antibody has also been reported for use in immunoprecipitation (IP). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for LAT Antibody (LAT.10-17) [Allophycocyanin]

  • 36 kDa phospho-tyrosine adapter protein
  • LAT
  • LAT1,36 kDa phospho-tyrosine adaptor protein
  • linker for activation of T cells
  • linker for activation of T-cells family member 1
  • p36-38
  • pp36

Background

This LAT.10-17 (10-17) monoclonal antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated signal transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream signaling molecules such as Grb-2, PLC?, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, natural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on LAT.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

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Bioinformatics

Gene Symbol LAT
Entrez