LAMP-2/CD107b Antibody (M3/84) [Biotin]

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Flow Cytometry: LAMP-2/CD107b Antibody (M3/84) [Biotin] [NB100-77527] - Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with biotinylated M3/84, followed by Sav-PE

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77527
    • Availability
      Product Discontinued

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LAMP-2/CD107b Antibody (M3/84) [Biotin] Summary

Immunogen
Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Marker
Late Endosome / Lysosome marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
LAMP2
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry <= 0.25 ug/10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application. Useful in Immunohistochemistry-Frozen and Immunoprecipitation as reported in Literature.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for LAMP-2/CD107b Antibody (M3/84) [Biotin]

  • CD107 antigen-like family member B
  • CD107b antigen
  • CD107b
  • LAMP2
  • LAMP-2
  • LAMPB
  • LGP110
  • lysosomal-associated membrane protein 2
  • lysosome-associated membrane glycoprotein 2
  • Lysosome-associated membrane protein 2

Background

Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for LAMP-2/CD107b Antibody (NB100-77527)

Discover related pathways, diseases and genes to LAMP-2/CD107b Antibody (NB100-77527). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for LAMP-2/CD107b Antibody (NB100-77527)

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Research Areas for LAMP-2/CD107b Antibody (NB100-77527)

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Blogs on LAMP-2/CD107b.

LAMP2 - a marker of lysosomes and late endosomes
Lysosomes are membrane-bound organelles responsible for the degradation of various biological macromolecules. Vesicles containing hydrolytic enzymes bud from the Golgi and fuse with endosomes to form the mature lysosome capable of breaking down va...  Read full blog post.

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Bioinformatics

Gene Symbol LAMP2
Entrez