Laminin beta 1 Antibody (DG10)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51757
    • Availability
      Product Discontinued

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Laminin beta 1 Antibody (DG10) Summary

Immunogen
The antibody reacts with human tissues.
Marker
Schwann Cell Marker
Specificity
The antibody recognizes the Mr 220000 b1-chain of laminin. Mab DG10 appears to react with the NH2-terminus of the Ln b1chain (1). The antibody reacts with human tissues. chains are useful in basic biology studies as well as in pathological studies concerning, for instance, the integrity of the basement membranes in carcinomas. Clone DG 10 against laminin beta 1-chain is apparently one of the few laminin antibodies working perfectly in immunoblotting, thus making it especially valuable for biochemical studies. Monoclonal antibody (Mab) to laminin (b1-chain) is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Partially pepsin-digested laminins from human placenta were used as immunogen (8).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Immunohistochemical stainings on frozen sections, western blotting and other immunoassays for cellular and tumor biology. Unlike many other Mabs to Ln b1chain Mab DG10 reacts strongly both under reducing and nonreducing conditions. Mab DG10 has been succe

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
100 mg immunoglobulin with 1 ml PBS and 1.0% (w/v) BSA
Preservative
0.1% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Laminin beta 1 Antibody (DG10)

  • CLM
  • cutis laxa with marfanoid phenotype
  • Laminin B1 chain
  • laminin subunit beta-1
  • laminin, beta 1
  • Laminin-1 subunit beta
  • Laminin-10 subunit beta
  • Laminin-12 subunit beta
  • Laminin-2 subunit beta
  • Laminin-6 subunit beta
  • Laminin-8 subunit beta
  • MGC142015

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kDa. It plays an important role in many aspects of the cell biology. Laminin is composed of one A chain (400 kDa) one B1 chain (215 kDa) and one B2 chain (205 kDa) all held together by disulfide bonds. The molecule has a cross like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells. Laminins from various species have common antigenic determinants. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. It has been found to modulate cell differentiation, cell shaping and also cell movement because it appears to be an important cell substrate adhesion protein. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. The greatest interest in laminin has been provoked by the discovery of its ability to promote neurite regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LAMB1