Laminin Antibody

Images

 
Immunohistochemistry Free-Floating: Laminin Antibody [NB300-144] - Immunohistological analysis of brain stem section stained with rabbit polyclonal Laminin Antibody [NB300-144], dilution 1:1,000 in red, and costained ...read more
Immunohistochemistry: Laminin Antibody [NB300-144] - MxIF analysis of mouse submandibular salivary gland morphogenesis. MxIF of a developmental TMA including embryonic stages (E14, E16, E18) and postnatal stages (P5 and ...read more
Immunohistochemistry Free-Floating: Laminin Antibody [NB300-144] - Staining of mouse section of cortex stained with Laminin Antibody [NB300-144] (red). Blue is DAPI staining of DNA. This antibody reveals strong staining ...read more
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using Laminin Antibody [NB300-144]. Pepsin antigen retrieval was performed on ...read more
Western Blot: Laminin Antibody [NB300-144] - Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) Laminin Antibody [NB300-144] recognizes 3 laminin ...read more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - IF Confocal analysis of HeLa cells using Laminin Antibody [NB300-144] (1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Western Blot: Laminin Antibody [NB300-144] - Western blot analysis of the effects of IP6, Ins, IP6 + Ins and normal saline on the levels of collagen IV, Laminin and Fibronectin, with Laminin Antibody [NB300-144]. IP6 or ...read more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in HeLa cells using Laminin Antibody [NB300-144] at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst ...read more
Immunohistochemistry: Laminin Antibody [NB300-144] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) using Laminin Antibody [NB300-144] shows vascular ...read more
Flow Cytometry: Laminin Antibody [NB300-144] - A surface stain was performed on HeLa cells with the Alexa Fluor 647 conjugate of Laminin Antibody [NB300-144AF647] (blue) and a matched isotype control (orange). Cells ...read more
Western Blot: Laminin Antibody [NB300-144] - Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right), using Laminin Antibody [NB300-144]. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, In, Ma, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Laminin Antibody Summary

Immunogen
Laminin Antibody was made to Laminin 111 isolated from mouse Engelbreth-Holm-Swarm (EHS) sarcoma cells. [UniProt# P19137]
Localization
Secreted, Basement membrane, Extracellular matrix
Marker
Basement Membrane Marker
Specificity
Laminin Antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMA1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:5000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Frozen 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunohistochemistry Free-Floating 1:1000 - 1:5000
Application Notes
This Laminin antibody detects bands at around 440, 220, and 158 kDa in Western Blot. Use in flow cytometry (PMID: 31819166) reported in scientific literature. Use in ICC/IF, IHC, IHC-Frozen, IHC-Paraffin, and Western Blot reported in multiple pieces of scientific literature. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue. br/>
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
337 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 8 Reviews rated 4.5
using
NB300-144 in the following applications:

Publications
Read Publications using
NB300-144 in the following applications:

  • 1 publication
  • ICC
    1 publication
  • 20 publications
  • IHC
    15 publications
  • 5 publications
  • 5 publications
  • KO
    1 publication
  • WB
    13 publications

Reactivity Notes

Rabbit, Fruit Bat, Chinese Hamster, and S. mansoni reactivity reported in scientific literature (PMID: 18214989, 31877588, 29251349, and 28114363 respectively). Human, Mouse, Rat, and Sheep reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
IgG purified

Alternate Names for Laminin Antibody

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • PTBHS
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-144
Species: Hu, Mu, Rt, ChHa, In, Ma, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl

Publications for Laminin Antibody (NB300-144)(63)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinese Hamster, Invertebrate, Mammal, Rabbit, Sheep.

We have publications tested in 8 applications: Flow, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB.


Filter By Application
Flow
(1)
ICC
(1)
ICC/IF
(20)
IHC
(15)
IHC-Fr
(5)
IHC-P
(5)
KO
(1)
WB
(13)
All Applications
Filter By Species
Human
(14)
Mouse
(27)
Rat
(12)
Chinese Hamster
(1)
Invertebrate
(1)
Mammal
(1)
Rabbit
(1)
Sheep
(3)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using NB300-144 Applications Species
Herrmann A, Lahtz C, Song J, et al. Integrin alpha 6 signaling induces STAT3-TET3-mediated hydroxymethylation of genes critical for maintenance of glioma stem cells Oncogene Dec 9 2019 [PMID: 31819166] (Flow, Human) Flow Human
Wu D, Witt RL, Harrington DA, Farach-Carson MC Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated alpha1 beta1 Integrin-Mediated Motility Front Cell Dev Biol Oct 16 2019 [PMID: 31750298] (ICC/IF, Human) ICC/IF Human
Kamynina A et al.. Furthering the understanding of the redox control of soluble Epoxide Hydrolase and Protein Kinase G in the cardiovascular system Thesis 2019 (IHC-Fr, Mouse)

Details:
Citation using the DyLight 488 format of this antibody.
IHC-Fr Mouse
Mosele FC, Bissi Ricci R, Abreu P, Rosa Neto JC Muscle regeneration in adiponectin knockout mice showed early activation of anti-inflammatory response with perturbations in myogenesis J. Cell. Physiol. Jan 31 2020 [PMID: 32003014] (IHC, Mouse) IHC Mouse
Britto F, Gnimassou O, De Groote E Acute environmental hypoxia potentiates satellite cell dependent myogenesis in response to resistance exercise through the inflammation pathway in human FASEB j. Dec 10 2019 [PMID: 31914659] (ICC/IF, Human) ICC/IF Human
Hanadhita D, Rahma A, Wahid MR et al. Extracellular matrix composition of different spleen compartments of fruit bats Anat Histol Embryol Dec 26 2019 [PMID: 31877588] (IHC, Mammal) IHC Mammal
Kunz L, Schroeder T A 3D Tissue-wide Digital Imaging Pipeline for Quantitation of Secreted Molecules Shows Absence of CXCL12 Gradients in Bone Marrow Cell Stem Cell Dec 5 2019 [PMID: 31809740] (ICC/IF, Mouse) ICC/IF Mouse
Cummins CB, Gu Y, Wang X et al. Burn-Induced Impairment of Ileal Muscle Contractility Is Associated with Increased Extracellular Matrix Components J. Gastrointest. Surg. 2019 Oct 21 [PMID: 31637625]
BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling Choi S, Yu J, Park A et al. Sci Rep [PMID: 31409851] (WB, Human) WB Human
Miska J, Lee-Chang C, Rashidi A et al. Miska J, Lee-Chang C, Rashidi A et al. Cell Rep [PMID: 30943404] (ICC/IF, Mouse) ICC/IF Mouse
Show All 63 Publications.

Reviews for Laminin Antibody (NB300-144) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB300-144:
Filter by Applications
WB
(3)
ICC
(3)
IHC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(5)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Laminin NB300-144
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reviewed by:
Anonymous
WB Human 03/07/2020
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-468 human breast cancer cell line
SpeciesHuman
Lot187-013019
Immunocytochemistry Laminin NB300-144
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reviewed by:
Anonymous
ICC Human 10/24/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedTM cells
SpeciesHuman
Lot196052516

Comments

Commentsuse at 1:100
Western Blot Laminin NB300-144
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reviewed by:
Anonymous
WB Human 10/24/2017
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Summary

ApplicationWestern Blot
Sample Testedhuman eye
SpeciesHuman
Lot196052516

Comments

Commentsuse at 1:500
Immunohistochemistry Laminin NB300-144
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reviewed by:
Anonymous
IHC Mouse 06/27/2017
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Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Western Blot Laminin NB300-144
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reviewed by:
Anonymous
WB Mouse 06/15/2017
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Summary

ApplicationWestern Blot
Sample TestedMouse cornea
SpeciesMouse
Immunocytochemistry Laminin NB300-144
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reviewed by:
Anony- mous
ICC Human 06/04/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF
Immunocytochemistry Laminin NB300-144
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reviewed by:
Gail Seigel
ICC Human 11/14/2012
View

Summary

ApplicationImmunocytochemistry
Sample TestedWERI-RB27 human retinoblastoma cells
SpeciesHuman
LotLot 1
CommentsThe antibody worked well on the first attempt

Blocking

Blocking DetailsAbcam protein block Ab64226, ready-to-use, 15 minutes at room temp.

Primary Anitbody

Dilution RatioLAMA1 1:200 dilution for one hour at room temp

Secondary Antibody

Secondary DescriptionTwo-step Polymer-HRP kit
Secondary Manufacturer Cat#Syd Laboratories PT03-D
Secondary Concentrationready-to-use

Details

Detection NotesDAB reagent for 5 minutes
Fixation DetailsCytospin preparations permeabilized with PBS-Tween for 5 minutes at room temp.
Wash DescriptionTPBS three times 5 minutes

Comments

CommentsThe antibody worked well on the first attempt
 
reviewed by:
Anonymous
IHC-Fr Rat 12/06/2011
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat
SpeciesRat

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Secondary Antibody

Secondary DescriptionSecondary Ab: Rabbit

Details

Detection NotesDetection Method: DAB

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin Antibody (NB300-144). (Showing 1 - 7 of 7 FAQs).

  1. What research areas can this product be used in?
    • All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
  2. What is the theoretical molecular weight for your Laminin antibodies?
    • The theoretical molecular weight for Laminin antibodies is 337 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  4. I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
    • The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.
  5. I have a question concerning the antibody NB300-144. The antibody blocks the function of the laminin. Can you tell me, which site of the laminin the antibody attacks? Because I need an antibody which block the binding from laminin to the integrin receptor by binding to the globular domain (integrin binding site) of the laminin.
    • Our bioassays show that this antibody binds to laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. In culture, the antibody hinders cell migration on a laminin substratum. It also inhibits neurite and cell process outgrowth. Applied to tissue sections, it inhibits neurite outgrowth by neurons seeded onto the tissue section. As this is a polyclonal antibody, it is nearly impossible to determine the specific antigen binding sites. No molecular level tests for laminin domain recognition have been performed. However, based on bioassays, it appears that the mix of antibodies in this purified polyclonal anti-serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited; that is, cell receptor binding is markedly hindered. It is the best, among many, laminin function-blocking preps we have tested. Most polyclonal antibodies raised in rabbit against a mouse laminin-1 will inhibit the activity of many rodent laminin isoforms. This one is especially specific, avid and purified for that purpose.
  6. Do you have any WB images tested by NB300-144 on human? I see the WB image on the datasheet, but I do not know what sample is loaded on each lane?
    • The samples used for the western blot image are Laminin-1 (LN-1) protein from EHS tumor and Laminin-2 (LN-2) protein from heart.
  7. What isoforms does this product react with?
    • Laminin Antibody [NB300-144] is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-144AF350
Alexa Fluor 405 NB300-144AF405
Alexa Fluor 488 NB300-144AF488
Alexa Fluor 532 NB300-144AF532
Alexa Fluor 594 NB300-144AF594
Alexa Fluor 647 NB300-144AF647
Alexa Fluor 700 NB300-144AF700
Alexa Fluor 750 NB300-144AF750
Allophycocyanin NB300-144APC
Allophycocyanin/Cy7 NB300-144APCCY7
Biotin NB300-144B
DyLight 350 NB300-144UV
DyLight 405 NB300-144V
DyLight 488 NB300-144G
DyLight 550 NB300-144R
DyLight 594 NB300-144DL594
DyLight 650 NB300-144C
DyLight 680 NB300-144FR
DyLight 755 NB300-144IR
FITC NB300-144F
HRP NB300-144H
Janelia Fluor 549 NB300-144JF549
Janelia Fluor 646 NB300-144JF646
PE NB300-144PE
PE/Atto594 NB300-144PEATT594
PE/Cy5.5 NB300-144PECY55
PE/Cy7 NB300-144PECY7
PerCP NB300-144PCP

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Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews

4.5
5
5
4
2
3
1
2
0
1
0

Anonymous
03/07/2020
Application: WB
Species: Human

Anonymous
10/24/2017
Application: ICC
Species: Human

Anonymous
10/24/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol LAMA1
Entrez
Uniprot