Lamin B2 Antibody (LN43)

Images

 
Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (LN43) [NBP1-97890] - Staining of a 9 days old zebrafish embryo
Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (LN43) [NBP1-97890] - Immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in the ductal epithelium

Product Details

Summary
Reactivity Hu, Mu, Po, Fi, XpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr
Clone
LN43
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lamin B2 Antibody (LN43) Summary

Immunogen
Clone LN43 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with the detergent insoluble fraction of potoroo cell line PtK1.
Marker
Nuclear Envelope Marker
Specificity
LN43 reacts with an epitope located in the Cterminal part of lamin B2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LMNB2
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Human, Mouse, Hamster, Porcine and Xenopus. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Lamin B2 Antibody (LN43)

  • lamin B2
  • lamin-B2
  • LMN2LAMB2
  • MGC2721

Background

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. For integrin subunits alpha 3 and alpha 6, two cytoplasmic variants, A and B, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lamin B2 Antibody (NBP1-97890). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?
    • We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LMNB2