LAG-3 Overexpression Lysate (Denatured)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

LAG-3 Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-LAG3 full-length
Gene
LAG3

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for LAG-3

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-LAG3 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LAG-3 Overexpression Lysate (Denatured)

  • CD223 antigen
  • CD223
  • FDC
  • LAG3
  • LAG-3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Protein FDC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for LAG-3 Lysate (H00003902-T01). (Showing 1 - 4 of 4 FAQ).

  1. What is the concentration of your biotin conjugated LAG-3 antibody?
    • We actuall do not determine the concentration of our conjugated antibodies but they are usuall 80-100% the concentration of the base product, so the concentration should be at least 0.8 mg/ml.
  2. Can this LAG-3 antibody be used in ELISA?
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  3. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.
  4. Is this LAG-3 antibody offered in a smaller size that is less expensive?
    • We do not offer a sample size for this particular LAG 3 antibody but we do offer another, AF3329, in a 25 ug sample size.

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Bioinformatics

Gene Symbol LAG3
Entrez