L1CAM Antibody (UJ127)

Images

 
Western Blot: L1CAM Antibody (UJ127) - Neuronal Marker [NB600-555] - L1CAM from Human cells.
Immunohistochemistry-Paraffin: L1CAM Antibody (UJ127) - Neuronal Marker [NB600-555] - Human schwanoma stained with L1 Cell Adhesion Molecule Ab-1 (LCAM) NB 600-555 using peroxidase-conjugate and AEC chromogen. Note ...read more
Western Blot: L1CAM Antibody (UJ127) - Neuronal Marker [NB600-555] - Native L1CAM immunoprecipitated from Human cells.

Product Details

Summary
Product Discontinued
View other related L1CAM Primary Antibodies

Order Details


    • Catalog Number
      NB600-555
    • Availability
      Product Discontinued

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L1CAM Antibody (UJ127) Summary

Immunogen
Homogenous suspension of 16 week human fetal brain
Localization
Cell membrane.
Marker
Axon Marker
Specificity
NB600-555 is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin. It does not bind to pediatric or adult brain.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
L1CAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 2 ug/mg
Application Notes
WB: Detects an approx. 220 - 240 kDa, which corresponds to the predicted molecular weight of L1CAM.
Reviewed Applications
Read 1 Review rated 4
using
NB600-555 in the following applications:

Publications
Read Publications using
NB600-555 in the following applications:

  • WB
    1 publication

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 20335502).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for L1CAM Antibody (UJ127)

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for L1CAM Antibody (NB600-555)(2)

Review for L1CAM Antibody (NB600-555) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB600-555:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Huafeng Zhang
WB Human 08/04/2010
View

Summary

ApplicationWestern Blot
Sample TestedMDA-231 cell lysate, Sample Amount: 100ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:200, Incubation Dilution Buffer: 5%milk in TBST, Incubation Time: 1.5hr, Incubation Temp:RT

Secondary Antibody

Secondary DescriptionSecondary Ab: mouse, Secondary Ab Dilution Ratio: 1:2500
Secondary Manufacturer Cat#SantaCruz

Details

Detection NotesDetection Method: ECL Plus

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for L1CAM Antibody (NB600-555). (Showing 1 - 1 of 1 FAQs).

  1. Hello. For the following product, could you let us know the concentration of sodium azide? #NB600-555 L1CAM Antibody (UJ127) http://www.novusbio.com/L1CAM-Antibody-UJ127_NB600-555.html Buffer: 10mM PBS [pH7.4] with 0.2% BSA Preservative: Sodium Azide
    • The L1CAM antibody with catalogue number NB600-555 is supplied in 10mM PBS pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for L1CAM Antibody (NB600-555)

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Blogs on L1CAM

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Contact Information

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Recent Reviews

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5
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4
1
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2
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1
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Huafeng Zhang
08/04/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol L1CAM
Entrez
Uniprot