L1CAM Antibody (2A6)

Images

 
Western Blot: L1CAM Antibody (2A6) [NBP2-02149] Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (2A6) [NBP2-02149] - Staining of COS7 cells.
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (2A6) [NBP2-02149] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: L1CAM Antibody (2A6) [NBP2-02149] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: L1CAM Antibody (2A6) [NBP2-02149] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.
Flow Cytometry: L1CAM Antibody (2A6) [NBP2-02149] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
2A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

L1CAM Antibody (2A6) Summary

Immunogen
Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
Marker
Axon Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
L1CAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2-4ug/mg

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for L1CAM Antibody (2A6)

  • antigen identified by monoclonal R1
  • CAML1
  • CAML1N-CAML1
  • CD171 antigen
  • CD171
  • HSAS
  • HSAS1
  • L1 cell adhesion molecule
  • L1CAM
  • MASA
  • MIC5
  • N-CAM-L1
  • NCAM-L1
  • neural cell adhesion molecule L1
  • S10
  • SPG1

Background

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol L1CAM
Entrez