Ku80/XRCC5 Antibody

Images

 
Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - analysis of HeLa cell lysate (30ug) using anti-Ku80 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody [NBP1-56408] - Human lung adenocarcinoma cell line A549.
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody [NBP1-56408] - Human Pancrease (Epithelial cells of acinus indicated with arrows), 4-8ug/ml.
Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - Titration: 1.25ug/ml, Positive Control: Jurkat cell lysate.
Western Blot: Ku80/XRCC5 Antibody [NBP1-56408] - Titration: 1 ug/ml Positive Control: Human pancreatic cancer cell line MiaPaca-2 and Panc-1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ku80/XRCC5 Antibody Summary

Immunogen
Synthetic peptides corresponding to XRCC5 (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; 80kDa)) The peptide sequence was selected from the C terminal of XRCC5. Peptide sequence GIT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XRCC5
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against XRCC5 and was validated on Western Blot and immunohistochemistry-P
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-56408 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Ku80/XRCC5 Antibody

  • 80kDa
  • ATP-dependent DNA helicase 2 subunit 2
  • ATP-dependent DNA helicase II 80 kDa subunit
  • CTC85
  • CTCBF
  • EC 3.6.4.-
  • FLJ39089
  • G22P2
  • KARP1
  • KARP-1
  • Ku80
  • Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
  • Ku86
  • Ku86DNA repair protein XRCC5
  • KUB2
  • NFIV
  • Thyroid-lupus autoantigen
  • TLAA
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
  • X-ray repair cross-complementing protein 5
  • X-ray repair, complementing defective, repair in Chinese hamster
  • XRCC5

Background

XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ku80/XRCC5 Antibody (NBP1-56408) (0)

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Review for Ku80/XRCC5 Antibody (NBP1-56408) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-56408:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Ku80/XRCC5 NBP1-56408
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reviewed by:
Anonymous
WB Human 10/07/2014
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Summary

ApplicationWestern Blot
Sample TestedHela Cell Lysate
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBS buffer

Primary Anitbody

Dilution Ratio1:800 diluted in PBS buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP conjugated secondary antibody (Biopioneer)
Secondary Manufacturer Cat#GAR21002-1
Secondary Concentration1:15000

Details

Detection NotesDeveloped using FemtoLucent Plus HRP Chemiluminescent Kit with a 1 min exposure

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ku80/XRCC5 Antibody (NBP1-56408) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ku80/XRCC5 Antibody (NBP1-56408)

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Recent Reviews

4
5
0
4
1
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0
2
0
1
0

Anonymous
10/07/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol XRCC5
Entrez
Uniprot