Ku80/XRCC5 Antibody (MEM-54)

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Immunocytochemistry/ Immunofluorescence: Ku80 Antibody (MEM-54) [NB110-55299] - Staining of HeLa human cervix carcinoma cell line using purified anti-Ku Antigen (MEM-54) (detection by Goat anti-mouse IgG2a Alexa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IP
Clone
MEM-54
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Ku80/XRCC5 Antibody (MEM-54) Summary

Immunogen
human whole T-lymphocytes
Specificity
The antibody MEM-54 reacts with Ku80, a 80 kDa subunit of Ku autoantigen (heterodimer of 72 and 87 kDa polypeptides). Ku autoantigen is involved in DNA repair and in V(D)J recombination through its ability to bind DNA double-strand breaks.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
XRCC5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunoprecipitation 1:50
Application Notes
Immunocytochemistry: Staining technique - PFA-fixation

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Ku80/XRCC5 Antibody (MEM-54)

  • 80kDa
  • ATP-dependent DNA helicase 2 subunit 2
  • ATP-dependent DNA helicase II 80 kDa subunit
  • CTC85
  • CTCBF
  • EC 3.6.4.-
  • FLJ39089
  • G22P2
  • KARP1
  • KARP-1
  • Ku80
  • Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
  • Ku86
  • Ku86DNA repair protein XRCC5
  • KUB2
  • NFIV
  • Thyroid-lupus autoantigen
  • TLAA
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
  • X-ray repair cross-complementing protein 5
  • X-ray repair, complementing defective, repair in Chinese hamster
  • XRCC5

Background

Ku Antigen is a heterodimer (of 72 and 87 kDa polypeptides) which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end-joining pathway. A DNA double-strand break is a major lesion that destroys the integrity of the DNA molecule. Such damage is introduced by ionizing radiation. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and the Ku-DNA-dependent protein kinase complex may have a role in those same processes. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle 2 full turns of DNA while encircling only the central 3-4 base pairs. Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features are well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation. Mouse cells deficient for Ku80 display a marked increase in chromosomal aberrations, including breakage, translocations, and aneuploidy. Despite the observed chromosome instabilities, Ku80 -/- mice have only a slightly earlier onset of cancer. Loss of p53 synergizes with Ku80 to promote tumorigenesis such that all Ku80 -/- and p53 -/- mice succumb to disseminated pro-B-cell lymphoma before 3 months of age. The p70/p80 complex binds to the ends of double-stranded DNA in a cell cycle-dependent manner, being associated with chromosomes of interphase cells, followed by complete dissociation from the condensing chromosomes in early prophase. Some patients with systemic lupus erythematosus produce very large amounts of autoantibodies to p70 and p80. The autoantibody has been found in patients with autoimmune thyroid disease (Graves disease) as well as in those with lupus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol XRCC5
Entrez