Ku80/XRCC5 Antibody (235-1)

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Product Details

Summary
Product Discontinued
View other related Ku80/XRCC5 Primary Antibodies

Order Details


    • Catalog Number
      NB100-78524
    • Availability
      Product Discontinued

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Ku80/XRCC5 Antibody (235-1) Summary

Immunogen
CEM human T-cell leukemia cells
Specificity
Mouse Monoclonal to Ku (235-1)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
XRCC5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Recommended Usage: Each lot of this antibody is quality control tested by Immunofluorescence staining. For immunofluorescence microscopy, suggested working dilution(s): Use a dilution range of 2-4 microgram/ml. For immunoprecipitation, this antibody can be used at 2-4 mug /1x107 cell equivalents. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Ku80/XRCC5 Antibody (235-1)

  • 80kDa
  • ATP-dependent DNA helicase 2 subunit 2
  • ATP-dependent DNA helicase II 80 kDa subunit
  • CTC85
  • CTCBF
  • EC 3.6.4.-
  • FLJ39089
  • G22P2
  • KARP1
  • KARP-1
  • Ku80
  • Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
  • Ku86
  • Ku86DNA repair protein XRCC5
  • KUB2
  • NFIV
  • Thyroid-lupus autoantigen
  • TLAA
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
  • X-ray repair cross-complementing protein 5
  • X-ray repair, complementing defective, repair in Chinese hamster
  • XRCC5

Background

Ku, is a heterodimeric DNA end binding complex composed of 70 KD and 80 kD subunits, serves as the DNA binding component of the DNA-dependent protein kinase (DNA-PK). Ku binds to double-stranded DNA breaks (DSBs), activates the catalytic subunit (DNA-PKcs) when DNA is bound, and is essential in DSB repair and V(D)J recombination. Clone 235-1 has been reported to bind the holoprotein (70KD and 80KD heterodimer) and both chains are need for the antigen to be recognized by this antibody. Clone 235-1 has been shown to be useful for Immunoprecipitation and Immunofluorescence staining. This clone is not suitable for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol XRCC5
Entrez