KMT2B Antibody

Images

 
Western Blot: KMT2B Antibody [NB600-254] - analysis of MLL4 in Jurkat cell lysate (30ug/lane) using anti-MLL4 antibody. The primary antibody was used at a dilution of 1:2000 in PBS, incubated for 3 hours at room ...read more
Chromatin Immunoprecipitation: KMT2B Antibody [NB600-254] - Chromatin Immunoprecipitation: KMT2B Antibody [NB600-254] - enriched DNA binding sites versus input reference DNA. A. 10 ug of this antibody was used to ...read more
Western Blot: KMT2B Antibody [NB600-254] - Detection of Human Trithorax 2 on HeLa whole cell lysate using NB600-254. Immunoprecipitated TRX2 was detected by WB using either NB600-254 or another rabbit anti-TRX2 antibody.
Proximity Ligation Assay: KMT2B Antibody [NB600-254]
Proximity Ligation Assay: KMT2B Antibody [NB600-254] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1 (NB100-287) and rabbit anti-human TRX2 (NB600-254). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

KMT2B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2375 and 2425 of human Trithorax 2 using the numbering given in entry NP_055542.1 (GeneID 9757).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLL4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 -1:5000
  • Chromatin Immunoprecipitation 10 ug
  • Immunoprecipitation 5 -10 ug/mg lysate
  • Proximity Ligation Assay 1:1000 - 1:10000
Application Notes
This antibody is useful for: Western Blot: 1:500 to 1:5,000, Immunoprecipitation: 5 to 10 mcg/mg lysate, Chromatin IP (ChIP): 1 to 5 mcg
Reviewed Applications
Read 1 Review rated 4
using
NB600-254 in the following applications:

Publications
Read Publications using
NB600-254 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KMT2B Antibody

  • EC 2.1.1.43
  • histone-lysine N-methyltransferase MLL4
  • HRX2
  • KIAA0304WW domain-binding protein 7
  • Lysine N-methyltransferase 2D
  • mixed lineage leukemia gene homolog 2
  • MLL2
  • myeloid/lymphoid or mixed-lineage leukemia 4
  • Myeloid/lymphoid or mixed-lineage leukemia protein 4
  • Trithorax homolog 2
  • trithorax homologue 2
  • TRX2
  • WBP7
  • WBP-7
  • WW domain binding protein 7

Background

This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KMT2B Antibody (NB600-254)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for KMT2B Antibody (NB600-254) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB600-254:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot KMT2B NB600-254
Enlarge
reviewed by:
Smita Singh
WB Human 12/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate from Jurkat cells grown in RPMI medium
SpeciesHuman

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 30 sec

Comments

CommentsMLL4 is degraded very fast and thats why this antibody and most others give some smaller bands.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for KMT2B Antibody (NB600-254) (0)

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Secondary Antibodies

 

Isotype Controls

Additional KMT2B Products

KMT2B NB600-254

Bioinformatics Tool for KMT2B Antibody (NB600-254)

Discover related pathways, diseases and genes to KMT2B Antibody (NB600-254). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for KMT2B Antibody (NB600-254)

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Pathways for KMT2B Antibody (NB600-254)

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PTMs for KMT2B Antibody (NB600-254)

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Research Areas for KMT2B Antibody (NB600-254)

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Blogs on KMT2B

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Smita Singh
12/17/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MLL4
Entrez
Uniprot