Klotho beta Antibody (1025C) [Alexa Fluor® 488]

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HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB58891G, filled histogram) or isotype control ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
1025C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 488

Klotho beta Antibody (1025C) [Alexa Fluor® 488] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse myeloma cell line, NS0-derived recombinant human Klotho beta with a C-terminal 10 His tag.
Phe53-Leu997
Accession # Q86Z14
Specificity
Detects human Klotho beta in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klotho beta Antibody (1025C) [Alexa Fluor® 488]

  • betaKlotho
  • beta-klotho
  • BKL
  • KLB
  • klotho beta like
  • Klotho beta
  • klotho beta-like protein
  • MGC142213

Background

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1, 2). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (3). The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-5). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2-4, 6). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 7). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (8). The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.

  1. Mian, I. S. (1998) Blood Cells Mol. Dis. 24:83.
  2. Kurosu, H. and M. Kuro-o (2009) Mol. Cell. Endocrinol. 299:72.
  3. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
  4. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  5. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
  6. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
  7. Chang, Q. et al. (2005) Science 310:490.
  8. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB58891U
Alexa Fluor 405 FAB58891V
Alexa Fluor 488 FAB58891G
Alexa Fluor 594 FAB58891T
Alexa Fluor 647 FAB58891R
Alexa Fluor 700 FAB58891N
Alexa Fluor 750 FAB58891S

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