KLF4 Antibody (1E5)

Images

 
Western Blot: KLF4 Antibody (1E5) [NBP1-51636] - Western blot analysis using KLF4 mouse mAb against KLF4 (aa2-470)-hIgGFc transfected HEK293 (1) and HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: KLF4 Antibody (1E5) [NBP1-51636] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and human rectum tissues (right) using KLF4 mouse mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
1E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

KLF4 Antibody (1E5) Summary

Immunogen
Purified recombinant fragment of human KLF4 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KLF4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for KLF4 Antibody (1E5)

  • Epithelial zinc finger protein EZF
  • EZF
  • EZFendothelial Kruppel-like zinc finger protein
  • GKLFKrueppel-like factor 4
  • Gut-enriched krueppel-like factor
  • KLF4
  • Kruppel-like factor 4 (gut)

Background

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for KLF4 Antibody (NBP1-51636)

Discover related pathways, diseases and genes to KLF4 Antibody (NBP1-51636). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on KLF4.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

KLF4 opens the door for stem cell research
KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi...  Read full blog post.

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Bioinformatics

Gene Symbol KLF4
Entrez