KIST Antibody (2H4)

Images

 
Western Blot: KIST Antibody (2H4) [NBP2-03027] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIST (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: KIST Antibody (2H4) [NBP2-03027] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KIST.
Immunohistochemistry-Paraffin: KIST Antibody (2H4) [NBP2-03027] - Staining of paraffin-embedded Human liver tissue using anti-KIST mouse monoclonal antibody.
Flow Cytometry: KIST Antibody (2H4) [NBP2-03027] - Analysis of Hela cells, using anti-KIST antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: KIST Antibody (2H4) [NBP2-03027] Analysis of extracts (35ug) from 9 different cell lines by using anti-KIST monoclonal antibody.
Immunohistochemistry-Paraffin: KIST Antibody (2H4) [NBP2-03027] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIST mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

KIST Antibody (2H4) Summary

Immunogen
Full length human recombinant protein of human UHMK1 (NP_787062) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UHMK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for KIST Antibody (2H4)

  • DKFZp434C1613
  • EC 2.7.11.1
  • FLJ23015
  • kinase interacting with leukemia-associated gene (stathmin)
  • Kinase interacting with stathmin
  • KISKIS protein kinase
  • KIST
  • serine/threonine-protein kinase Kist
  • U2AF homology motif (UHM) kinase 1
  • U2AF homology motif kinase 1

Background

The gene encodes a serine/threonine protein kinase that promotes cell cycle progression through G1 by phosphorylation of the cyclin-dependent kinase inhibitor 1B (p27Kip1), which causes nuclear export and degradation. The encoded protein is also thought to function in the adult nervous system and the gene has been associated with schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UHMK1
Entrez