Ki-67/MKI67 Antibody (4A1)

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Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (4A1) [NBP1-47538] - Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D), showing nuclear ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47538
    • Availability
      Product Discontinued

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Ki-67/MKI67 Antibody (4A1) Summary

Immunogen
Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MKI67
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1000
Application Notes
Immunohistochemistry-Paraffin, ELISA. Although not tested this antibody may be useful in in Immunohistochemistry-Frozen.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Ki-67/MKI67 Antibody (4A1)

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional Ki-67/MKI67 Products

Bioinformatics Tool for Ki-67/MKI67 Antibody (NBP1-47538)

Discover related pathways, diseases and genes to Ki-67/MKI67 Antibody (NBP1-47538). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Ki-67/MKI67 Antibody (NBP1-47538)

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PTMs for Ki-67/MKI67 Antibody (NBP1-47538)

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Research Areas for Ki-67/MKI67 Antibody (NBP1-47538)

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Blogs on Ki-67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is...  Read full blog post.

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Bioinformatics

Gene Symbol MKI67
Entrez