Keap1 Antibody (1B4)

Images

 
Western Blot: Keap1 Antibody (1B4) [NBP2-03319] - Analysis of human BEAS2B cells.
Immunohistochemistry-Paraffin: KEAP1 Antibody (1B4) [NBP2-03319] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
Flow Cytometry: KEAP1 Antibody (1B4) [NBP2-03319] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed ...read more
Western Blot: KEAP1 Antibody (1B4) [NBP2-03319] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Western Blot: KEAP1 Antibody (1B4) [NBP2-03319] Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody.
Western Blot: Keap1 Antibody (1B4) [NBP2-03319] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; ...read more
Western Blot: Keap1 Antibody (1B4) [NBP2-03319] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues used at a 1:200 dilution.
Immunohistochemistry-Paraffin: KEAP1 Antibody (1B4) [NBP2-03319] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: KEAP1 Antibody (1B4) [NBP2-03319] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody.
Flow Cytometry: KEAP1 Antibody (1B4) [NBP2-03319] - Analysis of Hela cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: KEAP1 Antibody (1B4) [NBP2-03319] - Analysis of Jurkat cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
1B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Keap1 Antibody (1B4) Summary

Immunogen
Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KEAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Publications
Read Publication using NBP2-03319.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Keap1 Antibody (1B4)

  • INrf2
  • INrf2MGC9454
  • Keap1
  • kelch-like ECH-associated protein 1
  • Kelch-like protein 19
  • KIAA0132MGC4407
  • KLHL19Cytosolic inhibitor of Nrf2
  • MGC10630
  • MGC1114
  • MGC20887

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC, IHC-P

Publications for Keap1 Antibody (NBP2-03319)(1)


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Bioinformatics

Gene Symbol KEAP1
Entrez