KDEL Antibody (2E7)

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Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
2E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

KDEL Antibody (2E7) Summary

Immunogen
Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
Localization
Endoplasmic Reticulum
Marker
Endoplasmic Reticulum Marker
Specificity
Detects approx 78kDa.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
POGLUT2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
1 ug/ml of HDEL Antibody was sufficient for detection of HDEL-containing proteins in 10 ug of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Fruit Fly (Drosophila melanogaster)PlantBarnyard Grass (Echinochloa)Beet (Beta vulgaris)Cotton (Gossypium)Mung Bean (Vigna radiata)Grass (Sorghum)Wheat (Triticum spp.).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for KDEL Antibody (2E7)

  • Endoplasmic reticulum resident protein 58
  • EP58KDEL1
  • ER protein 58
  • ERp58
  • KDEL (Lys-Asp-Glu-Leu) containing 1
  • KDEL motif-containing 1
  • KDEL motif-containing protein 1
  • MGC5302

Background

1 ug/mL was sufficient for detection of HDEL-containing proteins in 10ug of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Additional KDEL Products

Array NBP2-12885

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Bioinformatics

Gene Symbol POGLUT2