KCTD15 Antibody (4C3)

Images

 
Western Blot: KCTD15 Antibody (4C3) [NBP1-49429] - The cell lysates (10ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanKCTD15 antibody (1:1000). Proteins were visualized using agoat ...read more
Immunocytochemistry/ Immunofluorescence: KCTD15 Antibody (AT4C3) [NBP1-49429] - Human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 ...read more
Flow Cytometry: KCTD15 Antibody (4C3) [NBP1-49429] - Analysis of KCTD15 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype ...read more
Western Blot: KCTD15 Antibody (AT4C3) [NBP1-49429] - PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
4C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

KCTD15 Antibody (4C3) Summary

Immunogen
Recombinant human KCTD15 (1-234aa) purified from E. coli.
Specificity
Human KCTD15 (1-234aa)
Isotype
IgG3 Lambda
Clonality
Monoclonal
Host
Mouse
Gene
KCTD15
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:250

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for KCTD15 Antibody (4C3)

  • BTB/POZ domain-containing protein KCTD15
  • MGC25497
  • MGC2628
  • potassium channel tetramerisation domain containing 15

Background

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49429AF405
Alexa Fluor 488 Labeled NBP1-49429AF488
Alexa Fluor 647 Labeled NBP1-49429AF647
Alexa Fluor 700 Labeled NBP1-49429AF700
DyLight 350 Labeled NBP1-49429UV
DyLight 405 Labeled NBP1-49429V
DyLight 488 Labeled NBP1-49429G
DyLight 680 Labeled NBP1-49429FR
DyLight 755 Labeled NBP1-49429IR

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Bioinformatics

Gene Symbol KCTD15
Entrez
Uniprot