KAT3B/p300 Antibody

Product Discontinued

KAT3B/p300 Antibody Summary

• Immunogen: A synthetic peptide made to a portion of human E1A binding protein p300 encoded within exons 15 and 16 (LocusLink ID 2033).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EP300
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Sequencing
  • Immunohistochemistry
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:5000-1:25000
• Application Notes: By Immunoprecipitation, this antibody recognizes a band at ~300 kDa, representing p300. Also useful for Western Blot. *The investigator should determine the optimal working dilution for a specific application.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-1567, NB200-171, NB100-97831, NB100-58826, NB600-233, NB100-312, NB100-314

Alternate Names for KAT3B/p300 Antibody

• E1A binding protein p300
• E1A-associated protein p300
• E1A-binding protein, 300kD
• EC 2.3.1
• EC 2.3.1.48
• EP300
• histone acetyltransferase p300
• KAT3B
• p300 HAT
• p300
• RSTS2

Background

p300 is highly homologous to CREB-binding protein (CBP). Like CBP, it is a conserved transcriptional coactivator and histone acetyltransferase (HAT) that links chromatin remodeling with transcription. It also plays a role in cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KAT3B/p300 Antibody (NB500-160)(1)

Publication using NB500-160

• Zhang LJ, Vogel WK, Liu X et al. Coordinated regulation of transcription factor Bcl11b activity in thymocytes by the mitogen-activated protein kinase (MAPK) pathways and protein sumoylation. J Biol Chem 2012-08-01 [PMID: 22700985]

Bioinformatics

• Gene Symbol: EP300
• Entrez:
  • 2033(Human)