Jurkat Whole Cell Lysate

Product Discontinued

Jurkat Whole Cell Lysate Summary

• Description: This product is not supplied in loading buffer. If you intend to us this product in Western Blot we recommend 1 x sample buffer with 5% 2-Mercaptoethanol (BME) or other reducing agent.
• Specificity: Total Protein - Human Tumor Cell Line: Jurkat

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • SDS-Page
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Blood Lymph
• Subcellular Fraction: Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.