Jurkat Whole Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, In vitro, IP
Concentration
2 mg/ml

Order Details

Jurkat Whole Cell Lysate Summary

Description
Whole cell lysate (non-denatured)
Localization
T lymphocyte
Preparation
Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay.

Applications/Dilutions

Application Notes
In vitro assay usage reported in scientific literature (PMID: 23964713).
Publications
Read Publication using L017V2.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
In modified RIPA Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF).
Concentration
2 mg/ml

Lysate Details for Jurkat

Type
Cell
Tissue
Blood Lymph
Protein State
Native
Subcellular Fraction
Whole

Notes

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Jurkat Lysate (L017V2)(1)

We have publications tested in 1 application: In vitro.


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In vitro
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional Jurkat Products

Jurkat L017V2

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