JunB/AP-1 [p Ser259] Antibody

Images

 
Western Blot: JuNBPhosphospecific Antibody [NB100-92568] - Western blot (WB) analysis of p-JuNB(S259) antibody in extracts from HeLa cells.
Immunohistochemistry: JuNBPhosphospecific Antibody [NB100-92568] - Immunohistochemistry (IHC) analyzes of p-JuNB(S259) antibody in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92568
    • Availability
      Product Discontinued

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JunB/AP-1 [p Ser259] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human JunB around the phosphorylation site of Serine 259.
Modification
p Ser259
Specificity
p-JunB (S259) pAb detects endogenous levels of JunB protein only when phosphorylated at S259.
Clonality
Polyclonal
Host
Rabbit
Gene
JUNB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:500-1:200
Application Notes
Western blot (WB) analysis of p-JunB (S259) pAb in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of p-JunB (S259) pAb in paraffin-embedded human breast carcinoma tissue
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JunB/AP-1 [p Ser259] Antibody

  • activator protein 1
  • AP-1
  • jun B proto-oncogene
  • JunB
  • transcription factor jun-B

Background

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol JUNB
Entrez
Uniprot