JNK1 Antibody (1C2)

Images

 
Western Blot: JNK1 Antibody (1C2) [NBP1-48285] Analysis of extracts (35ug) from 9 different cell lines by using anti-JNK1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (1C2) [NBP1-48285] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1.
Western Blot: JNK1 Antibody (1C2) [NBP1-48285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Western Blot: JNK1 Antibody (1C2) [NBP1-48285] - JNK1 antibody ( 1C2 ) at 1:1000 + NIH/3T3 cell lysate

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-48285
    • Availability
      Product Discontinued

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JNK1 Antibody (1C2) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-384 of human JNK1
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a1.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAPK8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1000
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for JNK1 Antibody (1C2)

  • c-Jun N-terminal kinase 1
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK1 alpha protein kinase
  • JNK1 beta protein kinase
  • JNK1
  • JNK1JNK1A2
  • JNK-46
  • JUN N-terminal kinase
  • MAP kinase 8
  • MAPK 8
  • MAPK8
  • mitogen-activated protein kinase 8 isoform JNK1 alpha1
  • mitogen-activated protein kinase 8 isoform JNK1 beta2
  • mitogen-activated protein kinase 8
  • PRKM8
  • PRKM8JNK
  • protein kinase JNK1
  • SAPK1
  • SAPK1JNK21B1/2
  • Stress-activated protein kinase 1
  • Stress-activated protein kinase JNK1

Background

JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK1 is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of JNK1 by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. JNK1 is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of JNK1 gene suggested that it play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for JNK1 Antibody (NBP1-48285)

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Blogs on JNK1.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. Enc...  Read full blog post.

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Bioinformatics

Gene Symbol MAPK8
Entrez