JAM-A Antibody (OTI3H3)

Western Blot: JAM-A Antibody (OTI3H3) [NBP2-45560] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JAM-A.

Immunohistochemistry: JAM-A Antibody (OTI3H3) [NBP2-45560] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: JAM-A Antibody (OTI3H3) [NBP2-45560] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescence: JAM-A Antibody (OTI3H3) [NBP2-45560] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY JAM-A.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: OTI3H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

JAM-A Antibody (OTI3H3) Summary

• Immunogen: Full length human recombinant protein of human F11R(NP_653087) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: F11R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
• Theoretical MW: 29.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for JAM-A Antibody (OTI3H3)

• CD321 antigen
• CD321
• F11 receptor
• F11R
• JAM-1
• JAM1CD321
• JAMA
• JAM-A
• JCAMJAM
• Junctional adhesion molecule 1Platelet F11 receptor
• junctional adhesion molecule A
• PAM-1
• PAM-1KAT
• Platelet adhesion molecule 1

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAM-A Antibody (NBP2-45560)(1)

Publication using NBP2-45560

• Kobayashi K, Mochizuki J, Yamazaki F, Sashihara T Yogurt starter strains ameliorate intestinal barrier dysfunction via activating AMPK in Caco-2 cells Tissue barriers 2023-02-28 [PMID: 36852963] (Immunocytochemistry/ Immunofluorescence, Human)

Bioinformatics

• Gene Symbol: F11R