Jak2 Antibody

Images

 
Western Blot: Jak2 Antibody [NBP1-19651] - Analysis of JAK2 (R564) pAb at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Western Blot: Jak2 Antibody [NBP1-19651] - Analysis of JAK2 (R564) antibody in 293 etoposide 25uM 24h.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-19651
    • Availability
      Product Discontinued

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Jak2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 541-590 of Human JAK2.
Clonality
Polyclonal
Host
Rabbit
Gene
JAK2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
Western blot (WB) analysis of JAK2 (R564) pAb in 293 etoposide 25uM 24h.
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Jak2 Antibody

  • EC 2.7.10
  • EC 2.7.10.2
  • Jak2
  • JAK-2
  • Janus kinase 2Janus kinase 2 (a protein tyrosine kinase)
  • JTK10
  • tyrosine-protein kinase JAK2

Background

JAK2 (Janus Activating Kinase 2) is a tyrosine kinase of the non-receptor type, that associates with the intracellular domains of cytokine receptors; JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin; it has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Jak2 Antibody (NBP1-19651)

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Diseases for Jak2 Antibody (NBP1-19651)

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Research Areas for Jak2 Antibody (NBP1-19651)

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Blogs on Jak2.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

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Bioinformatics

Gene Symbol JAK2
Entrez