Isopeptidase T/USP5 Antibody (2H5)

Images

 
Western Blot: USP5 Antibody (2H5) [NBP2-02647] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.
Immunohistochemistry-Paraffin: USP5 Antibody (2H5) [NBP2-02647] - Staining of paraffin-embedded Human thyroid tissue using anti-USP5 mouse monoclonal antibody.
Western Blot: USP5 Antibody (2H5) [NBP2-02647] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: USP5 Antibody (2H5) [NBP2-02647] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-USP5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP5 Antibody (2H5) [NBP2-02647] - Staining of paraffin-embedded Human Kidney tissue using anti-USP5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP5 Antibody (2H5) [NBP2-02647] - Staining of paraffin-embedded Human pancreas tissue using anti-USP5 mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-02647
    • Availability
      Product Discontinued

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Isopeptidase T/USP5 Antibody (2H5) Summary

Immunogen
Full length human recombinant protein of human USP5 (NP_003472) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
USP5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Dog, Rat, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Isopeptidase T/USP5 Antibody (2H5)

  • Deubiquitinating enzyme 5
  • EC 3.1.2.15
  • EC 3.4.19.12
  • Isopeptidase T
  • IsoT
  • ISOTubiquitin carboxyl-terminal hydrolase 5
  • ubiquitin isopeptidase T
  • ubiquitin specific peptidase 5 (isopeptidase T)
  • ubiquitin specific protease 5 (isopeptidase T)
  • ubiquitin thioesterase 5
  • Ubiquitin thiolesterase 5
  • ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
  • Ubiquitin-specific-processing protease 5
  • USP5

Background

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol USP5
Entrez