Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein

Images

 
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein [NBP1-72523]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-414 of Human Isocitrate Dehydrogenase 1/IDH1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKL

Details of Functionality
Specific activity is > 18,000 pmol/min/ug. One unit will oxidize 1.0 pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
IDH1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein

  • Cytosolic NADP-isocitrate dehydrogenase
  • EC 1.1.1.42
  • IDCD
  • IDH
  • IDH1
  • IDP
  • IDPC
  • isocitrate dehydrogenase [NADP] cytoplasmic
  • isocitrate dehydrogenase 1 (NADP+), soluble
  • Isocitrate Dehydrogenase 1
  • NADP(+)-specific ICDH
  • NADP-dependent isocitrate dehydrogenase, cytosolic
  • NADP-dependent isocitrate dehydrogenase, peroxisomal
  • Oxalosuccinate decarboxylase
  • PICD

Background

IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22166
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-168
Species: Hu, Mu(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB110-61646
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-42210
Species: Hu
Applications: WB, Flow, IHC, IHC-P
AF2988
Species: Mu, Rt
Applications: WB
NBP2-32104
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
H00171023-M05
Species: Hu
Applications: WB, ELISA
NBP1-32259
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-32398
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-34980
Species: Hu
NBP1-54755
Species: Hu
Applications: WB
NBP1-87069
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB120-13888
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IHC-P, CyTOF-ready, IHC-WhMt
NBP1-26612
Species: Hu
Applications: IP (-), WB
NBP2-45978
Species: Hu
Applications: WB, Flow, IHC
NB600-1438
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IP
NBP1-72523
Species: Hu
Applications: Func, PAGE

Publications for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523) (0)

There are no publications for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523) (0)

There are no reviews for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Isocitrate Dehydrogenase 1/IDH1 Products

Bioinformatics Tool for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523)

Discover related pathways, diseases and genes to Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523)

Discover more about diseases related to Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523).
 

Pathways for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523)

View related products by pathway.

PTMs for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523)

Learn more about PTMs related to Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523).
 

Research Areas for Isocitrate Dehydrogenase 1/IDH1 Recombinant Protein (NBP1-72523)

Find related products by research area.

Blogs on Isocitrate Dehydrogenase 1/IDH1

There are no specific blogs for Isocitrate Dehydrogenase 1/IDH1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol IDH1
Entrez